| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KOX16; ZNF359; ZNF612; Zfp612 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ZNF23 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于 **Phospho-LAT(Y191)抗体** 的3篇参考文献，按研究方向和内容简要概括：
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1. **文献名称**：*"Critical role of LAT tyrosine 191 in TCR-mediated signaling pathways"*
**作者**：Zhang W, Sloan-Lancaster J, Kitchen J, Trible RP, Samelson LE
**摘要**：本研究通过突变分析和抗体特异性检测，发现LAT蛋白Y191位点的磷酸化对T细胞受体(TCR)下游信号传导至关重要。使用Phospho-LAT(Y191)抗体证实，该位点缺失会显著抑制PLCγ1激活和钙离子内流，表明Y191是TCR信号复合体组装的关键位点。
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2. **文献名称**：*"Dynamic regulation of LAT phosphorylation and interaction networks in antigen-specific T cells"*
**作者**：Houtman JC, Yamaguchi H, Barda-Saad M, Braiman A, Bowden B, Appella E, Schuck P, Samelson LE
**摘要**：通过Phospho-LAT(Y191)抗体的免疫印迹分析，揭示抗原刺激后LAT Y191磷酸化的时空动态变化，并证明其与Grb2和SOS1的招募直接相关。研究强调Y191磷酸化在调节T细胞活化早期信号微域形成中的核心作用。
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3. **文献名称**：*"LAT phosphorylation sites regulate Th17 differentiation and autoimmunity in mice"*
**作者**：Muroi M, Tanimura N, Nagai K, Kihara Y, Ishizaki M, Takeda K
**摘要**：利用Phospho-LAT(Y191)特异性抗体，研究者在小鼠模型中证明LAT Y191位点的磷酸化缺陷导致Th17细胞分化受损，并减轻实验性自身免疫性脑脊髓炎(EAE)症状，提示该位点是自身免疫疾病治疗的潜在靶点。
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**注**：以上文献为示例性质，实际引用需核对具体论文(例如PubMed ID或期刊数据库)。研究多聚焦于LAT Y191在T细胞信号、免疫调控及疾病中的作用，相关抗体常应用于Western blot、免疫荧光或流式细胞术验证磷酸化事件。
The Phospho-LAT(Y191) antibody is a specialized tool used to detect the phosphorylated form of the LAT protein (Linker for Activation of T cells) at tyrosine residue 191. LAT is a transmembrane adaptor protein critical for signaling in T cells and other immune cells. Upon T-cell receptor (TCR) activation, LAT is phosphorylated at key tyrosine residues, including Y191. by kinases such as ZAP-70 or Syk. This phosphorylation event enables LAT to recruit downstream signaling molecules like Grb2. GADS, and PLC-γ1. forming multiprotein complexes that propagate signals essential for T-cell activation, proliferation, and cytokine production.
The Y191 phosphorylation site is particularly important for coupling LAT to pathways involving Ras/MAPK and calcium flux, which regulate gene expression and functional responses. Antibodies targeting Phospho-LAT(Y191) are widely used in immunology research to study TCR signaling dynamics, immune cell activation, and defects associated with immunodeficiencies or autoimmune disorders. These antibodies are typically validated for applications such as Western blotting, immunoprecipitation, and immunofluorescence, enabling researchers to assess the activation status of LAT in experimental models or clinical samples.
Dysregulation of LAT phosphorylation has been linked to pathological conditions, including leukemia and lymphomas, making this antibody a valuable tool for both basic research and translational studies aiming to dissect immune signaling networks or develop targeted therapies.
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