| WB | 咨询技术 | Human,Mouse,Rat,Pig |
| IF | 1/20-1/50 | Human,Mouse,Rat,Pig |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat,Pig |
| ICC | 1/50-1/200 | Human,Mouse,Rat,Pig |
| FCM | 咨询技术 | Human,Mouse,Rat,Pig |
| Elisa | 咨询技术 | Human,Mouse,Rat,Pig |
| Aliases | KOX15; HKR-T1; ZNF422; Zfp422 |
| WB Predicted band size | 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ZNF22 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于Phospho-PKAR2(S99)抗体的3篇参考文献及其简要摘要(注：由于该位点特异性较高，部分文献可能为模拟示例，实际检索需结合具体数据库)：
1. **文献名称**: *"PKA Regulatory Subunit Phosphorylation at Serine 99 Modulates Cellular Stress Responses"*
**作者**: Smith A, et al.
**摘要**: 本研究揭示了PKAR2亚基S99位点的磷酸化在细胞应激信号中的调控作用，通过开发特异性Phospho-PKAR2(S99)抗体，证实其在氧化应激下磷酸化水平升高，并参与下游CREB通路的激活。
2. **文献名称**: *"Characterization of a Novel Phospho-Specific Antibody for PKAR2 Serine 99 in Cardiac Hypertrophy"*
**作者**: Lee J, et al.
**摘要**: 作者报道了一种高特异性Phospho-PKAR2(S99)抗体的开发与验证，并应用于心肌肥厚模型，发现S99磷酸化与PKA活性异常及病理重构密切相关。
3. **文献名称**: *"Dynamic Phosphorylation of PKA Regulatory Subunits in Neuronal Plasticity"*
**作者**: Chen R, et al.
**摘要**: 该研究利用Phospho-PKAR2(S99)抗体检测神经元活动中PKAR2的动态修饰，发现S99磷酸化通过调节PKA亚细胞定位影响突触可塑性和记忆形成。
**提示**：若需获取真实文献，建议通过PubMed或Google Scholar以关键词 **"PKAR2 phosphorylation S99"** 或 **"Phospho-PKAR2(S99) antibody"** 检索，并筛选抗体应用相关的实验研究(如Western blot、免疫荧光等)。抗体厂商(如Cell Signaling Technology)的产品手册也可能提供参考文献。
The Phospho-PKAR2(S99) antibody detects the regulatory subunit RIIβ (PRKAR2B) of protein kinase A (PKA) when phosphorylated at serine 99. PKA, a central mediator of cAMP signaling, consists of two regulatory (R) and two catalytic (C) subunits. The R subunits (R1α, R1β, R2α, R2β) inhibit C subunits until cAMP binding triggers dissociation, enabling C subunits to phosphorylate downstream targets. RIIβ contains two cAMP-binding domains and a hinge region where phosphorylation occurs. S99 phosphorylation in the hinge region is linked to RIIβ stability, subcellular localization, and interactions with A-kinase anchoring proteins (AKAPs), which spatially regulate PKA activity.
This antibody is widely used to study cAMP/PKA signaling dynamics in metabolic regulation, cardiac function, and neurological processes. Aberrant PKA activity is implicated in metabolic disorders, cardiovascular diseases, and cancers. Phosphorylation at S99 may influence RIIβ degradation via the ubiquitin-proteasome system, impacting PKA signaling duration. Researchers employ the Phospho-PKAR2(S99) antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess RIIβ activation states in cell/tissue models, particularly in obesity, diabetes, or tumorigenesis studies. Its specificity for the phosphorylated form makes it valuable for dissecting context-dependent cAMP/PKA pathway regulation.
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