| WB | 咨询技术 | Human,Mouse,Rat,Cow,Dog |
| IF | 1/20-1/50 | Human,Mouse,Rat,Cow,Dog |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat,Cow,Dog |
| ICC | 1/50-1/200 | Human,Mouse,Rat,Cow,Dog |
| FCM | 1/20-1/100 | Human,Mouse,Rat,Cow,Dog |
| Elisa | 咨询技术 | Human,Mouse,Rat,Cow,Dog |
| Aliases | KOX12 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ZNF19 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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1. **"MEK1 mutations confer resistance to MEK and B-Raf inhibition"**
- **Authors**: Emery CM, et al.
- **摘要**: 该研究通过结构分析和功能实验，揭示了MEK1基因突变导致对MEK抑制剂和B-Raf抑制剂耐药的分子机制，并利用MEK1特异性抗体验证突变蛋白的表达及活性变化。
2. **"Characterization of a novel MEK1 antibody for immunohistochemical detection in cancer tissues"**
- **Authors**: Lee HJ, et al.
- **摘要**: 开发了一种高特异性的MEK1抗体，用于癌症组织免疫组化检测，验证其在结直肠癌和黑色素瘤中的表达水平，并与患者预后相关性分析。
3. **"Targeting MEK1 in RAS-mutant leukemia: Preclinical efficacy and biomarker analysis"**
- **Authors**: Chen SH, et al.
- **摘要**: 研究通过MEK1抗体检测白血病模型中MEK/ERK信号通路活性，评估MEK抑制剂在RAS突变型白血病的治疗效果，并确定磷酸化MEK1作为潜在生物标志物。
4. **"Structural basis of MEK1 activation by BRAF kinase"**
- **Authors**: Lavoie H, et al.
- **摘要**: 利用MEK1抗体和冷冻电镜技术解析BRAF激酶与MEK1的复合物结构，阐明BRAF如何通过磷酸化激活MEK1.为靶向药物设计提供结构依据。
MEK1 (Mitogen-Activated Protein Kinase Kinase 1), also known as MAP2K1. is a central component of the RAS/RAF/MEK/ERK signaling pathway, which regulates critical cellular processes including proliferation, differentiation, survival, and apoptosis. As a dual-specificity kinase, MEK1 phosphorylates and activates ERK1/2 (Extracellular Signal-Regulated Kinases 1 and 2), which subsequently translocate to the nucleus to modulate gene expression. Dysregulation of this pathway, often due to mutations in MEK1 or upstream regulators like RAS and RAF, is implicated in various cancers, inflammatory diseases, and developmental disorders. For example, activating MEK1 mutations (e.g., P124S, Q56P) are found in melanoma, lung adenocarcinoma, and cardio-facio-cutaneous syndrome.
MEK1 antibodies are essential tools in biomedical research for detecting and quantifying MEK1 protein expression, phosphorylation status, and localization in cells or tissues. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Phospho-specific MEK1 antibodies, which recognize activated forms (e.g., phosphorylated at Ser217/221), help assess pathway activity in response to stimuli or therapeutic interventions. The development of selective MEK1 antibodies has also supported the study of MEK inhibitors (e.g., trametinib, cobimetinib) in cancer therapy and resistance mechanisms. Researchers rely on these antibodies to validate MEK1 expression in disease models, monitor drug efficacy, and explore MEK1's role in signaling crosstalk. Ensuring antibody specificity through validation (e.g., knockout controls) is critical to avoid cross-reactivity with homologous proteins like MEK2.
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