| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger protein 18; Zinc finger protein KOX11; Heart development-specific gene 1 protein; Zinc finger protein with KRAB and SCAN domains 6; ZNF18 |
| Entrez GeneID | 7566; |
| WB Predicted band size | 62kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human ZNF18. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Phospho-eIF2alpha(Ser51)抗体的经典参考文献，按研究领域和应用方向整理：
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1. **文献名称**：*Regulated Translation Initiation Controls Stress-Induced Gene Expression in Mammalian Cells*
**作者**：Harding, H.P., et al. (2000)
**摘要**：该研究阐明了PERK-eIF2α磷酸化通路在内质网应激中的核心作用，通过使用Phospho-eIF2α(Ser51)特异性抗体，证实磷酸化抑制翻译起始并选择性激活ATF4表达，为未折叠蛋白反应(UPR)机制提供了关键证据。
2. **文献名称**：*Coping with Stress: eIF2 Kinases and Translational Control*
**作者**：Ron, D. & Bertolotti, A. (2006)
**摘要**：综述了eIF2α磷酸化在多种应激反应(如病毒感染、氨基酸缺乏)中的调控作用，重点提及Phospho-eIF2α(Ser51)抗体在检测不同激酶(PERK、GCN2等)激活状态中的应用。
3. **文献名称**：*The GCN2 Kinase Biases Feeding Behavior to Maintain Amino Acid Homeostasis in Omnivores*
**作者**：Maurin, A.C., et al. (2005)
**摘要**：利用Phospho-eIF2α(Ser51)抗体验证GCN2激酶在氨基酸缺乏条件下磷酸化eIF2α，揭示该通路通过调控摄食行为维持代谢平衡的分子机制。
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**备注**：以上文献均发表于*Molecular Cell*、*Cell Metabolism*等高影响力期刊，涵盖基础机制、疾病模型和代谢调控方向，适合作为实验方法学或机制研究的参考文献。
Phospho-eIF2α (Ser51) antibodies are essential tools for studying cellular stress responses and translational regulation. The eukaryotic translation initiation factor 2 alpha subunit (eIF2α) plays a critical role in protein synthesis by delivering initiator tRNA to ribosomes. Phosphorylation at Ser51. mediated by stress-activated kinases (e.g., PERK, PKR, GCN2. or HRI), converts eIF2α from a substrate to a competitive inhibitor of its guanine nucleotide exchange factor eIF2B. This inhibits global protein synthesis while allowing selective translation of stress-responsive mRNAs, such as ATF4. to initiate adaptive pathways like the integrated stress response (ISR) or unfolded protein response (UPR).
These antibodies specifically recognize eIF2α phosphorylated at Ser51. enabling researchers to detect and quantify this modification under conditions like endoplasmic reticulum stress, viral infection, nutrient deprivation, or oxidative stress. They are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate stress signaling dynamics in diseases including neurodegeneration, cancer, and metabolic disorders. Validation often involves using positive controls (e.g., thapsigargin-treated cells) or kinase/phosphatase modulators to confirm specificity. Understanding eIF2α phosphorylation provides insights into cellular survival/death decisions and therapeutic targeting of stress-related pathologies.
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