| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PDLIM5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PDLIM5抗体的参考文献示例(注：以下内容为模拟虚构，实际文献需通过学术数据库查询确认)：
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1. **文献名称**：*PDLIM5 Antibody Characterization and Its Role in Neuronal Signaling*
**作者**：Smith A, et al.
**摘要**：本研究验证了一种新型PDLIM5多克隆抗体的特异性，通过Western blot和免疫荧光确认其在人脑组织中的表达定位。结果表明，PDLIM5在神经元突触中富集，可能与精神疾病相关的信号通路有关。
2. **文献名称**：*Expression Profiling of PDLIM5 in Cardiac Tissues Using a Monoclonal Antibody Approach*
**作者**：Yamamoto T, et al.
**摘要**：开发了一种针对PDLIM5的单克隆抗体，用于研究其在心肌细胞中的表达模式。免疫组化显示PDLIM5在心脏肥大模型中表达上调，提示其参与病理性心肌重塑的调控。
3. **文献名称**：*PDLIM5 as a Biomarker in Depression: Antibody-Based Detection in Serum Samples*
**作者**：Chen L, et al.
**摘要**：利用商业PDLIM5抗体检测抑郁症患者血清中的蛋白水平，发现PDLIM5表达与疾病严重程度呈正相关，支持其作为潜在生物标志物的可能性。
4. **文献名称**：*Development of a Phospho-Specific PDLIM5 Antibody for Cancer Research*
**作者**：Kumar R, et al.
**摘要**：研究团队开发了一种磷酸化PDLIM5的特异性抗体，证实其在多种癌细胞系中识别磷酸化位点的能力，为研究PDLIM5在肿瘤转移中的作用提供了工具。
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建议通过PubMed、Google Scholar或Web of Science等平台，以关键词“PDLIM5 antibody”、“PDLIM5 detection”等检索真实文献。
The PDLIM5 antibody is a research tool designed to detect and analyze the PDZ and LIM domain-containing protein 5 (PDLIM5), also known as enigma homolog (ENH). PDLIM5 is a scaffolding protein involved in cytoskeletal organization, signal transduction, and cellular compartmentalization. It features a PDZ domain at its N-terminus and LIM domains at the C-terminus, enabling interactions with various proteins, including ion channels, kinases, and transcription factors. PDLIM5 is expressed in multiple tissues, with notable roles in the brain, heart, and skeletal muscle. Dysregulation of PDLIM5 has been implicated in neuropsychiatric disorders (e.g., bipolar disorder, schizophrenia), cardiovascular diseases, and cancer, making it a target for mechanistic studies.
The PDLIM5 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study protein expression, localization, and interactions. Researchers employ it to explore PDLIM5's involvement in cellular processes such as neuronal synaptic plasticity, cardiac hypertrophy, and tumor progression. Commercial antibodies are typically validated for specificity against human, mouse, or rat PDLIM5 isoforms. However, users must verify cross-reactivity and experimental conditions due to potential isoform diversity and post-translational modifications. Its applications span basic research, biomarker discovery, and therapeutic target validation, reflecting PDLIM5's multifaceted biological significance.
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