| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NSMCE1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NSMCE1抗体的3篇参考文献及其摘要概括：
1. **"Structural basis of SMC5/6 complex functions in DNA repair"**
- **作者**: Li et al. (2020)
- **摘要**: 本研究解析了SMC5/6复合体的冷冻电镜结构，揭示了NSMCE1亚基在复合体组装和DNA损伤修复中的关键作用。研究通过特异性抗体验证了NSMCE1与其他亚基的互作，并证明其在维持基因组稳定性中的必要性。
2. **"NSMCE1 suppresses genome instability by restricting recombination at telomeres"**
- **作者**: Silva et al. (2019)
- **摘要**: 文章利用NSMCE1抗体进行免疫荧光和染色质免疫沉淀实验，发现NSMCE1通过抑制端粒区域异常重组防止染色体断裂，缺失该蛋白会导致端粒功能障碍和细胞衰老。
3. **"The SUMO ligase activity of the SMC5/6 complex is required for DNA repair in Arabidopsis"**
- **作者**: Huang et al. (2018)
- **摘要**: 研究在植物模型中通过NSMCE1抗体检测蛋白表达水平，证实其SUMO连接酶活性对同源重组修复至关重要，并揭示了该功能在跨物种间的保守性。
(注：以上文献信息为模拟示例，实际引用时需核对真实数据库及原文。)
The NSMCE1 antibody targets the NSMCE1 protein (also known as NSE1), a critical subunit of the SMC5/6 (Structural Maintenance of Chromosomes 5/6) complex. This evolutionarily conserved complex plays essential roles in maintaining genomic stability, including DNA repair, replication fork restart, and chromosome segregation. Specifically, NSMCE1 contributes to the E3 ubiquitin ligase activity of the SMC5/6 complex, facilitating post-translational modifications required for DNA damage response and homologous recombination repair. Dysregulation of NSMCE1 has been linked to genomic instability disorders and cancer progression.
The NSMCE1 antibody is widely used in research to investigate the protein's expression, localization, and functional interactions. It enables detection via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding studies on SMC5/6 complex dynamics in cellular responses to DNA damage. Researchers also employ this antibody to explore NSMCE1's role in diseases, particularly its tumor-suppressive functions and potential as a therapeutic target. Validation of antibody specificity remains crucial, as cross-reactivity with unrelated proteins could lead to misinterpretation of experimental results. Recent studies highlight NSMCE1's involvement in viral defense mechanisms, further expanding its biological significance.
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