| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/100-1/200 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NEDD4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NEDD4抗体的3篇参考文献的简要信息，涵盖其在不同研究中的应用和功能验证：
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1. **文献名称**: *NEDD4-1 is a proto-oncogenic ubiquitin ligase for PTEN*
**作者**: Wang, X., Trotman, L.C., Koppie, T., et al.
**摘要**: 本研究证实NEDD4通过泛素化降解肿瘤抑制因子PTEN促进癌症发展。研究使用特异性NEDD4抗体进行免疫共沉淀(Co-IP)和Western blot分析，揭示了NEDD4在多种癌细胞系中调控PTEN蛋白稳定性的机制。
2. **文献名称**: *Regulation of the endocytic adaptor protein ARH by NEDD4-family E3 ubiquitin ligases*
**作者**: Gallagher, E., Gao, M., Liu, Y.C., et al.
**摘要**: 文章探讨NEDD4家族成员对内吞衔接蛋白ARH的泛素化调控。通过NEDD4抗体进行免疫荧光和蛋白质定位分析，发现NEDD4L在神经元中特异性调控ARH的膜运输过程，影响细胞信号转导。
3. **文献名称**: *NEDD4 controls intestinal stem cell homeostasis by regulating the Hippo pathway*
**作者**: Bae, S.J., Kim, M., Kim, S.H., et al.
**摘要**: 研究利用NEDD4抗体在小鼠肠道类器官模型中验证其表达，发现NEDD4通过泛素化修饰Hippo通路核心激酶LATS1.调控肠道干细胞增殖与分化，为肠道再生及癌症治疗提供新靶点。
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以上文献展示了NEDD4抗体在癌症机制、神经元功能及干细胞研究中的关键应用，涵盖分子互作验证、亚细胞定位及疾病模型分析。如需具体DOI或发表年份，可进一步补充查询。
The NEDD4 (Neural Precursor Cell Expressed Developmentally Downregulated 4) antibody is a critical tool for studying the NEDD4 protein, a member of the HECT-type E3 ubiquitin ligase family. NEDD4 regulates ubiquitination, a post-translational modification that tags proteins for degradation or functional modulation. It contains a C2 domain (mediating membrane binding), multiple WW domains (substrate recognition), and a HECT catalytic domain. NEDD4 is involved in diverse cellular processes, including membrane receptor internalization, ion channel regulation, and apoptosis. It ubiquitinates substrates such as PTEN, EGFR, and Notch, influencing cancer progression, neuronal development, and immune responses.
Research using NEDD4 antibodies often focuses on its dual role in disease. In cancers, NEDD4 can act as an oncogene (e.g., promoting EGFR signaling in lung cancer) or tumor suppressor (e.g., degrading MYCN in neuroblastoma), depending on context. Dysregulation is also linked to neurodegenerative diseases (e.g., Alzheimer’s) and viral infections (e.g., HIV-1 budding). Antibodies targeting specific epitopes (e.g., N-terminal or HECT domain) enable detection of NEDD4 expression levels, subcellular localization, and interactions via techniques like Western blot, immunohistochemistry, and co-immunoprecipitation. Their applications extend to evaluating NEDD4 as a therapeutic target, particularly in diseases driven by aberrant protein ubiquitination. Validation of antibody specificity remains essential due to homology with other HECT ligases like NEDD4L.
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