| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human OBI1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是假设的关于OBI1抗体的参考文献示例(请注意，实际文献需根据具体数据库检索确认)：
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1. **文献名称**: "OBI1 interacts with BRCA1 and promotes homologous recombination repair in breast cancer"
**作者**: Zhang Y, et al.
**摘要**: 本研究通过免疫共沉淀和Western blot技术，证实OBI1抗体可用于检测其与BRCA1蛋白的相互作用，并发现OBI1通过调控同源重组修复途径抑制乳腺癌基因组不稳定性。
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2. **文献名称**: "Loss of OBI1 expression correlates with chemoresistance in ovarian carcinoma"
**作者**: Smith R, et al.
**摘要**: 利用OBI1抗体进行免疫组化分析，发现卵巢癌组织中OBI1蛋白表达缺失与铂类化疗耐药性显著相关，提示其作为治疗响应预测标志物的潜力。
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3. **文献名称**: "OBI1 regulates oxidative stress-induced apoptosis via modulation of p53 activity"
**作者**: Brown K, et al.
**摘要**: 通过siRNA敲低和OBI1抗体介导的蛋白质检测，揭示OBI1通过调控p53的磷酸化状态影响氧化应激下的细胞凋亡，为肿瘤治疗提供新靶点。
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4. **文献名称**: "Antibody-based profiling of OBI1 in triple-negative breast cancer identifies its prognostic value"
**作者**: Johnson L, et al.
**摘要**: 采用OBI1抗体对三阴性乳腺癌组织芯片进行染色，发现高表达OBI1的患者无病生存期显著延长，提示其作为预后生物标志物的临床价值。
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注：以上内容为示例性虚构，实际文献需通过PubMed、Google Scholar等平台检索确认。如需真实文献，请提供更具体的OBI1蛋白背景或研究领域。
The OBI1 antibody is a specialized immunological tool designed to target and detect the OBI1 protein, a member of the POU-domain transcription factor family. OBI1. also known as Oct-binding protein 1. plays a critical role in regulating gene expression by binding to octamer motifs (ATGCAAAT) in DNA. It is closely associated with cellular processes such as differentiation, proliferation, and maintenance of pluripotency in stem cells. First identified in studies exploring transcriptional regulation in early development, OBI1 is structurally characterized by a conserved POU domain that enables DNA binding and protein interaction.
Research involving OBI1 antibodies has been pivotal in elucidating the protein's involvement in embryonic development, tissue-specific gene activation, and oncogenesis. For instance, OBI1 is implicated in certain cancers, where dysregulation of its expression correlates with tumor progression or suppression. The antibody is widely used in techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to study OBI1 localization, expression levels, and DNA-binding activity.
Recent studies also highlight its potential as a biomarker in diagnostic or therapeutic contexts. However, specificity validation remains essential, as cross-reactivity with related POU-domain proteins (e.g., OCT1/OCT2) can occur. Overall, the OBI1 antibody serves as a vital reagent in molecular biology and oncology research, advancing understanding of transcriptional control mechanisms in health and disease.
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