| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APN; CD13; LAP1; P150; PEPN; GP150 |
| Entrez GeneID | 290 |
| clone | 1C7D7 |
| WB Predicted band size | 110kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ANPEP (AA: Extra(781-967)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于MAP4K5抗体的3篇文献示例(注：以下内容为假设性描述，实际文献需通过数据库检索确认)：
1. **文献名称**：*MAP4K5 regulates hepatic lipid metabolism through JNK signaling*
**作者**：Li X, et al.
**摘要**：该研究利用MAP4K5特异性抗体，发现其在高脂饮食诱导的小鼠非酒精性脂肪肝中表达升高，并通过调控JNK通路影响肝脏脂质沉积，提示MAP4K5可能成为代谢性肝病的干预靶点。
2. **文献名称**：*MAP4K5 inhibition enhances autophagy in Alzheimer’s disease models*
**作者**：Wang Y, et al.
**摘要**：通过Western blot和免疫荧光技术(使用MAP4K5抗体)，作者证明抑制MAP4K5可激活自噬通路，减少Tau蛋白异常磷酸化，为神经退行性疾病的治疗提供了新思路。
3. **文献名称**：*MAP4K5 promotes tumor metastasis via ERK/MMP9 axis in colorectal cancer*
**作者**：Chen Z, et al.
**摘要**：该研究通过免疫组化(MAP4K5抗体)分析结直肠癌组织，发现MAP4K5高表达与患者预后不良相关，机制上通过ERK/MMP9通路促进肿瘤侵袭转移。
---
**提示**：实际文献需通过PubMed、Google Scholar等平台以关键词“MAP4K5 antibody”或“MAP4K5 + 疾病/通路”检索，并关注抗体应用场景(如Western blot、IHC等)。
The MAP4K5 antibody is a crucial tool for studying the mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), a serine/threonine kinase belonging to the Ste20 family. Also known as germinal center kinase (GCK) or hematopoietic progenitor kinase 4 (HPK4), MAP4K5 regulates stress-activated signaling pathways, including the JNK and p38 MAPK cascades. It plays roles in diverse cellular processes such as apoptosis, inflammation, metabolism, and immune responses, with implications in diseases like cancer, diabetes, and cardiovascular disorders.
MAP4K5 antibodies are typically monoclonal or polyclonal reagents designed to detect endogenous MAP4K5 protein in applications like Western blotting, immunohistochemistry (IHC), immunoprecipitation (IP), and immunofluorescence (IF). They target specific epitopes, often in the kinase domain or C-terminal region, and are validated for species reactivity (e.g., human, mouse, rat). High-quality antibodies demonstrate minimal cross-reactivity with related kinases, ensuring specificity.
Research using MAP4K5 antibodies has elucidated its role in insulin resistance, autophagy, and tumor progression. For example, MAP4K5 inhibition has been explored as a therapeutic strategy for metabolic diseases. These antibodies are vital for quantifying MAP4K5 expression levels, studying post-translational modifications, and identifying interaction partners, making them essential for both basic research and drug development targeting MAP4K5-associated pathways.
×
