| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | Tilp; Psti-1; Spink1 |
| WB Predicted band size | 9 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of Rat Spink1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与SPINK1抗体相关的参考文献及其简要摘要：
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1. **标题**: *"SPINK1 promotes pancreatic cancer progression by activating EGFR signaling"*
**作者**: Li S, et al.
**摘要**: 研究通过免疫组化及Western blot分析，利用SPINK1抗体证实其在胰腺癌组织中高表达，并揭示其通过激活EGFR通路促进肿瘤侵袭的机制。
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2. **标题**: *"Autoantibody against SPINK1 as a novel biomarker for early detection of chronic pancreatitis"*
**作者**: Witt H, et al.
**摘要**: 通过ELISA和蛋白质芯片技术，发现慢性胰腺炎患者血清中SPINK1自身抗体水平显著升高，提示其可作为早期诊断标志物。
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3. **标题**: *"Immunohistochemical analysis of SPINK1 in premalignant pancreatic lesions"*
**作者**: Ohmuraya M, et al.
**摘要**: 采用SPINK1特异性抗体进行免疫组化染色，显示胰腺上皮内瘤变(PanIN)中SPINK1表达异常，与胰腺癌发生风险相关。
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4. **标题**: *"Development of a monoclonal SPINK1 antibody for targeted therapy in cystic fibrosis"*
**作者**: Keil M, et al.
**摘要**: 研究报道了一种新型SPINK1单克隆抗体的开发，通过体外实验验证其抑制胰蛋白酶异常活性的潜力，为囊性纤维化治疗提供新策略。
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这些文献涵盖了SPINK1抗体在疾病机制、诊断标志物开发及治疗应用中的研究。如需具体文章信息，可进一步通过PubMed或期刊数据库检索。
The SPINK1 antibody targets serine peptidase inhibitor Kazal type 1 (SPINK1), a protein encoded by the *SPINK1* gene. SPINK1. also known as pancreatic secretory trypsin inhibitor (PSTI), functions primarily to inhibit trypsin activity in the pancreas, preventing premature protease activation and tissue damage. It is expressed in pancreatic acinar cells and other tissues, including the prostate, liver, and gastrointestinal tract. Clinically, SPINK1 is linked to pancreatic disorders; mutations in the *SPINK1* gene are associated with hereditary and chronic pancreatitis. Moreover, SPINK1 overexpression is observed in several cancers (e.g., prostate, breast, and hepatocellular carcinomas), where it may promote tumor progression by modulating protease activity, cell proliferation, or apoptosis pathways.
SPINK1 antibodies are critical tools in research and diagnostics. They enable detection of SPINK1 expression via techniques like immunohistochemistry (IHC), Western blotting, or ELISA, aiding in studies of its role in disease mechanisms. Monoclonal antibodies offer high specificity for quantitative assays, while polyclonal antibodies may capture diverse epitopes in localization studies. Clinically, these antibodies help identify SPINK1 as a biomarker for pancreatitis risk stratification or cancer prognosis. Recent therapeutic strategies also explore neutralizing SPINK1 antibodies to block its oncogenic signaling. However, challenges remain in standardizing assays due to SPINK1's complex interactions and isoforms. Overall, SPINK1 antibodies are pivotal in unraveling the protein's pathophysiological roles and translational potential.
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