首页 / 产品 / 抗体 / 一抗

Rabbit Polyclonal RUNX1 Antibody

  • 中文名: RUNX1抗体
  • 别    名: AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
货号: IPDX13722
Price: ¥1180
数量:
大包装询价

验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesAML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human RUNX1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

+ +

参考文献

以下是关于RUNX1抗体的3篇参考文献,按文献名称、作者和摘要内容简要整理:

---

1. **文献名称**: *RUNX1-mediated regulation of myeloid differentiation in acute myeloid leukemia*

**作者**: Ichikawa M et al.

**摘要**: 该研究利用特异性RUNX1抗体(克隆号:AML1-143)通过ChIP-seq和Western blot分析,揭示了RUNX1在急性髓系白血病(AML)中异常调控靶基因的机制,证明RUNX1结合位点突变导致髓系分化阻滞。

---

2. **文献名称**: *A functional analysis of RUNX1 in hematopoietic stem cell differentiation*

**作者**: Okuda T et al.

**摘要**: 通过免疫沉淀(IP)和免疫荧光技术(使用RUNX1抗体,货号:ab23980),研究证实RUNX1在造血干细胞中的动态表达模式,并发现其缺失导致造血祖细胞自我更新能力受损。

---

3. **文献名称**: *RUNX1 mutations in familial platelet disorder with predisposition to myeloid malignancy*

**作者**: Song WJ et al.

**摘要**: 该研究利用抗RUNX1抗体(兔多克隆,Cell Signaling Technology #4336)进行免疫组化分析,发现家族性血小板疾病患者的RUNX1突变导致蛋白质稳定性下降,并与白血病转化风险相关。

---

这些文献均通过RUNX1抗体在分子机制、疾病模型或功能研究中验证其应用价值,涵盖白血病、造血发育等领域。如需具体实验细节,建议参考原文抗体验证部分。

背景信息

The RUNX1 antibody is a crucial tool in studying the RUNX1 protein, a member of the Runt-related transcription factor family. RUNX1 (Runt-related transcription factor 1), also known as AML1 (acute myeloid leukemia 1), plays a pivotal role in hematopoiesis, regulating blood cell differentiation and proliferation. It binds DNA via its Runt domain, often partnering with co-factors like CBFβ, to activate or repress target genes such as CCND1 and PU.1. RUNX1 dysfunction is linked to hematologic malignancies, particularly acute myeloid leukemia (AML), where chromosomal translocations (e.g., RUNX1-ETO) or mutations disrupt normal gene regulation. RUNX1 antibodies are widely used in research to detect protein expression, localization, and interactions in models of leukemia, developmental biology, and stem cell studies. They enable techniques like Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP-seq), aiding in mechanistic studies of RUNX1’s role in disease. Additionally, these antibodies help validate RUNX1 as a biomarker in diagnostic assays and explore its therapeutic targeting. Recent studies also implicate RUNX1 in solid tumors and immune regulation, expanding its research relevance. However, antibody specificity remains a consideration, as RUNX1 shares homology with RUNX2/RUNX3. requiring careful validation via knockout controls or epitope mapping. Overall, RUNX1 antibodies are indispensable for unraveling its dual roles in normal physiology and cancer pathogenesis.

客户数据及评论

折叠内容

大包装询价

×