| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ICF; ICF1; M.HsaIIIB |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human DNMT3B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于DNMT3B抗体的参考文献及摘要概括：
1. **"Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases"**
- **作者**: Okano, M., et al. (1998)
- **摘要**: 该研究首次克隆并鉴定了哺乳动物DNMT3家族成员(包括DNMT3B)，通过特异性抗体验证了其蛋白在胚胎干细胞中的表达模式，并证明DNMT3B在发育过程中调控DNA甲基化的关键作用。
2. **"DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development"**
- **作者**: Robertson, K.D., et al. (2001)
- **摘要**: 研究利用特异性抗体(包括针对DNMT3B的多克隆抗体)进行免疫沉淀和Western blot分析，揭示了Dnmt3a和Dnmt3B在胚胎发育中调控基因组甲基化的协同功能。
3. **"The DNA methyltransferase DNMT3B maps to human chromosome 20q13 and is widely expressed in human tissues"**
- **作者**: Hansen, R.S., et al. (1999)
- **摘要**: 通过制备特异性抗DNMT3B抗体，研究者定位了该基因在人类染色体上的位置，并在多种组织中检测其蛋白表达水平，为研究DNMT3B在疾病中的异常表达提供工具。
以上文献均涉及DNMT3B抗体的开发、验证或应用，涵盖基因功能、表达分析和甲基化机制研究。如需更多文献或具体应用场景，可进一步补充说明。
DNMT3B (DNA methyltransferase 3B) is a critical enzyme involved in establishing de novo DNA methylation patterns during embryonic development and cellular differentiation. As part of the DNMT family, it works alongside DNMT3A and the cofactor DNMT3L to catalyze the transfer of methyl groups to cytosine residues in CpG dinucleotides, a process essential for regulating gene expression, genomic imprinting, and maintaining chromosomal stability. DNMT3B is particularly vital during early embryogenesis and germ cell maturation, where dynamic DNA methylation reshapes the epigenome.
Antibodies targeting DNMT3B are widely used in epigenetic research to study its expression, localization, and functional interactions. These tools enable detection via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Given the structural similarities between DNMT3B and DNMT3A, high-specificity antibodies are crucial to avoid cross-reactivity. Researchers often validate these antibodies using knockout cell lines or siRNA-mediated knockdown to confirm target specificity.
Dysregulation of DNMT3B is linked to developmental disorders (e.g., Immunodeficiency-Centromeric instability-Facial anomalies syndrome, ICF) and cancers, where aberrant methylation contributes to oncogenesis. DNMT3B antibodies thus play a key role in exploring disease mechanisms and therapeutic targets in epigenetics. Commercial DNMT3B antibodies are typically raised against epitopes in its N-terminal regulatory or C-terminal catalytic domains, with validation data critical for experimental reliability.
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