| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TF; TFA; CD142 |
| WB Predicted band size | 33 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human F3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及CKMT1A/CKMT1B抗体的相关文献摘要(经简化整理)：
1. **文献名称**: *Differential expression of mitochondrial creatine kinase isoforms in neuroblastoma cells*
**作者**: Wyss M, et al.
**摘要**: 研究利用特异性抗体区分CKMT1A和CKMT1B，发现二者在神经细胞中具有组织特异性分布，CKMT1A在轴突线粒体富集，可能与能量转运相关。
2. **文献名称**: *Characterization of the human CKMT1A and CKMT1B gene promoters*
**作者**: Haas RC, et al.
**摘要**: 通过亚型特异性抗体证实，CKMT1A在心脏和骨骼肌中高表达，而CKMT1B在脑组织中占主导，启动子区域差异导致表达调控不同。
3. **文献名称**: *Mitochondrial creatine kinase in cancer progression*
**作者**: Xu X, et al.
**摘要**: 使用CKMT1B特异性抗体发现，其在乳腺癌细胞线粒体中显著上调，促进ATP转移并与化疗耐药性相关，提示其作为潜在治疗靶点。
注：上述文献信息为模拟示例，实际引用需核对真实出版物。建议通过PubMed或Web of Science以关键词“CKMT1 isoform antibodies”检索最新研究。
CKMT1A (creatine kinase mitochondrial 1A) and CKMT1B (creatine kinase mitochondrial 1B) are isoforms of the mitochondrial creatine kinase (MtCK) family, encoded by the *CKMT1* gene through alternative splicing. These enzymes play a critical role in cellular energy metabolism, particularly in tissues with high energy demands such as cardiac muscle, skeletal muscle, and the brain. They catalyze the reversible transfer of a phosphate group between creatine and ATP, maintaining energy homeostasis by buffering intracellular ATP levels. CKMT1A and CKMT1B differ in their N-terminal sequences due to alternative splicing, though their functional roles in the phosphocreatine shuttle system overlap significantly.
Antibodies targeting CKMT1A/CKMT1B are essential tools for studying mitochondrial bioenergetics, cellular stress responses, and diseases linked to energy dysregulation. These antibodies enable the detection, localization, and quantification of CKMT1 isoforms in tissues or cell lysates via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research applications include investigating their roles in conditions such as cardiomyopathy, neurodegenerative disorders (e.g., Alzheimer’s), and cancer, where altered CKMT1 expression correlates with metabolic reprogramming. Additionally, CKMT1A/CKMT1B antibodies help explore mitochondrial dysfunction in aging and ischemia-reperfusion injury. Their specificity is validated using knockout controls or siRNA-mediated silencing to ensure accurate identification of target isoforms in complex biological samples.
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