| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human OSM |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HDAC7抗体的3篇参考文献示例(注：文献为虚构概括，仅用于示例格式)：
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1. **文献名称**：*HDAC7 regulates endothelial cell migration via transcriptional control of cytoskeletal dynamics*
**作者**：Wang Y, et al.
**摘要**：本研究利用HDAC7特异性抗体，通过免疫荧光和Western blot分析了HDAC7在血管内皮细胞中的亚细胞定位，发现其通过调控细胞骨架相关基因的表达影响迁移能力。
2. **文献名称**：*Phosphorylation-dependent nuclear export of HDAC7 in T-cell apoptosis*
**作者**：Dequiedt F, et al.
**摘要**：通过磷酸化特异性HDAC7抗体，揭示了HDAC7在T细胞凋亡过程中的核质转运机制，并证实其与14-3-3蛋白的相互作用依赖特定激酶信号通路。
3. **文献名称**：*HDAC7 interacts with PPARγ to regulate adipocyte differentiation*
**作者**：Choi JH, et al.
**摘要**：使用HDAC7抗体进行染色质免疫沉淀(ChIP)实验，证明HDAC7与PPARγ协同调控脂肪生成相关基因的转录抑制，影响前脂肪细胞分化。
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**说明**：以上文献为示例性质，实际引用时需查询真实数据库(如PubMed)并核实作者及摘要内容。如需具体文献，建议通过关键词“HDAC7 antibody”或“HDAC7 immunohistochemistry”在学术引擎中检索。
Histone deacetylase 7 (HDAC7) is a class IIa HDAC enzyme involved in epigenetic regulation by removing acetyl groups from histone proteins, leading to chromatin condensation and transcriptional repression. Unlike class I HDACs, HDAC7 shuttles between the nucleus and cytoplasm, modulating gene expression in coordination with cellular signaling pathways. It plays critical roles in development, differentiation, and disease processes, including cardiovascular formation, T-cell regulation, and cancer progression. HDAC7 interacts with transcription factors like MEF2 and FOXP3. influencing cell survival, metabolism, and immune responses.
HDAC7 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques such as Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP). Due to high homology among class IIa HDACs, antibody specificity is crucial; validation often involves knockout controls or siRNA-mediated HDAC7 depletion. Commercial HDAC7 antibodies typically target unique epitopes within its N-terminal regulatory domain or C-terminal catalytic region.
Research applications include investigating HDAC7's role in tumorigenesis, where it may act as an oncogene or tumor suppressor depending on context, and exploring its therapeutic potential as a target for HDAC inhibitors. Antibodies also aid in studying post-translational modifications (e.g., phosphorylation) that regulate HDAC7 activity. Proper validation ensures reliable detection of the ~104 kDa protein across human, mouse, and rat samples.
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