| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CT80; HILI; mili; PIWIL1L |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human PIWIL2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于PIWIL2抗体的参考文献概览(基于公开研究内容整理，具体引用请核实原文)：
1. **文献名称**: "The PIWI homologs HIWI and HILI in human cancers"
**作者**: Qiao D, et al.
**摘要**: 该研究利用PIWIL2抗体(HIWI)通过免疫组化分析发现，PIWIL2在多种癌症(如乳腺癌、结肠癌)的肿瘤干细胞中异常高表达，提示其可能作为癌症干细胞标志物及治疗靶点。
2. **文献名称**: "A novel class of small RNAs in mouse spermatogenic cells"
**作者**: Sasaki T, et al.
**摘要**: 通过PIWIL2特异性抗体进行免疫沉淀和Western blot分析，揭示了PIWIL2在小鼠睾丸生殖细胞中与piRNA复合物的相互作用，参与精子发生过程中的基因沉默机制。
3. **文献名称**: "PIWIL2 regulates ribosomal RNA biogenesis in germline stem cells"
**作者**: Lee JH, et al.
**摘要**: 研究使用PIWIL2抗体进行免疫荧光定位，发现其在果蝇生殖干细胞中调控核糖体RNA合成，影响干细胞自我更新和分化平衡。
4. **文献名称**: "Epigenetic silencing of PIWIL2 in human tumors"
**作者**: Tan L, et al.
**摘要**: 通过抗体验证发现，PIWIL2在肺癌和前列腺癌中因DNA甲基化而下调，其表达缺失与患者预后不良相关，提示其抑癌基因潜在功能。
注：以上为示例性内容，实际文献需通过PubMed等数据库检索确认。
The PIWIL2 antibody is a research tool designed to detect and study the PIWI-like protein 2 (PIWIL2), a member of the evolutionarily conserved Argonaute/PIWI protein family. PIWIL2. also known as HILI in humans, plays critical roles in germ cell development, stem cell self-renewal, and post-transcriptional gene regulation, particularly through its interaction with piwi-interacting RNAs (piRNAs). These small non-coding RNAs guide PIWIL2 to silence transposable elements, maintain genomic stability, and regulate epigenetic modifications during gametogenesis and early embryogenesis.
PIWIL2 antibodies are widely used in molecular biology and clinical research to investigate protein expression patterns, subcellular localization, and functional mechanisms. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence, helping researchers map PIWIL2's tissue-specific distribution, particularly in reproductive tissues, embryonic stem cells, and certain cancers. Aberrant PIWIL2 expression has been implicated in tumorigenesis, with studies linking its overexpression to testicular, breast, and gastrointestinal cancers, suggesting potential roles as a diagnostic biomarker or therapeutic target.
These antibodies are crucial for exploring PIWIL2's dual roles in both physiological processes (e.g., spermatogenesis) and pathological conditions, providing insights into reproductive disorders and cancer biology. Validation parameters including species reactivity, clonality, and application-specific performance are essential considerations for experimental reliability.
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