首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠谷胱甘肽过氧化物酶，线粒体(GPX4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPX4含量。GPX4作为一个重要靶点，是谷胱甘肽过氧化物酶家族成员。它能催化还原型谷胱甘肽清除脂质过氧化物，在调控细胞铁死亡过程中有关键作用。目前对其研究聚焦在通过调节GPX4活性干预铁死亡，用于肿瘤等疾病治疗。试剂盒检测范围为4.68 μU/mL-300 μU/mL，试剂盒适用于科研领域，如探究肝脏疾病、神经退行性疾病或代谢综合征等模型中GPX4活性变化，或用于抗氧化药物筛选及铁死亡机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gpx4Phospholipid hydroperoxide glutathione peroxidase ELISA kit; PHGPx ELISA kit; EC 1.11.1.12 ELISA kit; Glutathione peroxidase 4 ELISA kit; GPx-4 ELISA kit; GSHPx-4 ELISA kit
缩写：GPX4
Uniprot No.：P36970
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：4.68 μU/mL-300 μU/mL
灵敏度：1.17 μU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GPX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:400 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:800 | Average % | 87 | ||||
| Range % | 83-91 | |||||
| 1:1600 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of rat GPX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 83 | 80-86 | ||||
| EDTA plasma (n=4) | 92 | 87-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μU/mlOD1OD2AverageCorrected3002.5882.5972.5932.4391502.2922.2122.2522.098751.6141.6351.6251.47137.51.0211.0651.0430.88918.750.6220.6380.6300.4769.370.3840.3740.3790.2254.680.2420.2520.2470.09300.1520.1560.154 | ||||||
货期：3-5 working days
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