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产品描述：人谷胱甘肽过氧化物酶，线粒体(GPX4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPX4含量。GPX4是一种重要的靶点。它是谷胱甘肽过氧化物酶家族成员，在调节细胞内氧化还原平衡中起关键作用。研究发现其异常与多种疾病如癌症、神经退行性疾病等相关，深入探究其作用机制，有望为开发治疗这些疾病的新疗法提供方向。试剂盒检测范围为7.8 μU/mL-500 μU/mL，适用于基础科研中评估细胞或动物模型中GPX4的活性变化，例如研究氧化损伤相关疾病（如神经退行性疾病、肿瘤等）的分子机制，或用于抗氧化药物筛选及线粒体功能调控等实验。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Glutathione peroxidase 4 ELISA Kit; GPX 4 ELISA Kit; GPX-4 ELISA Kit; GPX4 ELISA Kit; GPX4_HUMAN ELISA Kit; GSHPx-4 ELISA Kit; MCSP ELISA Kit; mitochondrial ELISA Kit; PHGPx ELISA Kit; Phospholipid hydroperoxidase ELISA Kit; Phospholipid hydroperoxide glutathione peroxidase ELISA Kit; Phospholipid hydroperoxide glutathione peroxidase mitochondrial ELISA Kit; snGPx ELISA Kit; snPHGPx ELISA Kit; Sperm nucleus glutathione peroxidase ELISA Kit
缩写：GPX4
Uniprot No.：P36969
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 μU/mL-500 μU/mL
灵敏度：1.95 μU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GPX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 92 | |||||
| Range % | 86-97 | ||||||
| 1:2000 | Average % | 97 | |||||
| Range % | 92-103 | ||||||
| 1:4000 | Average % | 94 | |||||
| Range % | 88-98 | ||||||
| 1:8000 | Average % | 90 | |||||
| Range % | 85-95 | ||||||
回收率：
| The recovery of human GPX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 88-96 | |||||
| EDTA plasma (n=4) | 94 | 91-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| μU/mlOD1OD2AverageCorrected5002.4772.4402.4592.3252502.1012.1232.1121.9781251.6881.6541.6711.53762.51.0661.1021.0840.95031.20.6510.6840.6680.53415.60.3790.3600.3700.2367.80.2720.2550.2640.13000.1390.1280.134 | |||||||
货期：3-5 working days
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