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产品描述：人谷胱甘肽过氧化物酶3(GPX3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPX3含量。GPX3（谷胱甘肽过氧化物酶 3）是一种抗氧化酶。其背景在于它能保护细胞免受氧化应激损伤。研究机制上，它可催化谷胱甘肽还原过氧化物，降解过氧化氢等有害物质，维持细胞内氧化还原稳态，在多种疾病如癌症、心血管病等研究中受关注。试剂盒检测范围为15.6 μIU/mL-1000 μIU/mL，适用于氧化应激机制研究、疾病模型评估及生物标志物筛选等科研领域，可满足体外检测不同来源样本的需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Extracellular glutathione peroxidase ELISA Kit; Glutathione peroxidase 3 (plasma) ELISA Kit; Glutathione peroxidase 3 ELISA Kit; GPX 3 ELISA Kit; GPx P ELISA Kit; GPx-3 ELISA Kit; GPx-P ELISA Kit; GPX3 ELISA Kit; GPX3_HUMAN ELISA Kit; GPXP ELISA Kit; GSHPx 3 ELISA Kit; GSHPx P ELISA Kit; GSHPx-3 ELISA Kit; GSHPx-P ELISA Kit; Plasma glutathione peroxidase 3 ELISA Kit; Plasma glutathione peroxidase ELISA Kit
缩写：GPX3
Uniprot No.：P22352
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 μIU/mL-1000 μIU/mL
灵敏度：3.9 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GPX3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 93 | ||||
| Range % | 89-95 | |||||
| 1:2000 | Average % | 104 | ||||
| Range % | 100-107 | |||||
| 1:4000 | Average % | 92 | ||||
| Range % | 89-96 | |||||
| 1:8000 | Average % | 98 | ||||
| Range % | 95-103 | |||||
回收率：
| The recovery of human GPX3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-98 | ||||
| EDTA plasma (n=4) | 96 | 94-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| µIU/mlOD1OD2AverageCorrected 10002.6302.5662.5982.458 5002.3302.2242.2772.137 2501.7361.7041.7201.580 1251.0001.0521.0260.886 62.50.6220.6080.6150.475 31.250.3510.3690.3600.220 15.60.2340.2220.2280.088 00.1420.1380.140 | ||||||
货期：3-5 working days
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