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产品描述：人磷脂酰肌醇蛋白聚糖5(GPC5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、腹水样本中的GPC5含量。GPC5 即磷脂酰肌醇蛋白聚糖 -5，是定位于细胞膜表面的糖蛋白。在研究机制上，它可调控细胞信号传导、细胞增殖与迁移。在多种癌症中异常表达，有望成抗癌治疗靶点，但相关研究还需深入，以充分明确其作用和应用潜力。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础医学研究中GPC5蛋白表达的动态监测，例如肿瘤微环境研究、神经发育机制探索或代谢性疾病模型中GPC5的功能分析，为体外实验样本的蛋白水平检测提供可靠工具，所有应用仅限于科学研究领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：bA93M14.1 ELISA Kit; glypican 5 ELISA Kit; Glypican 5 precursor ELISA Kit; glypican proteoglycan 5 ELISA Kit; Glypican-5 ELISA Kit; GPC5 ELISA Kit; GPC5_HUMAN ELISA Kit; Secreted glypican-5 ELISA Kit
缩写：GPC5
Uniprot No.：P78333
种属：Homo sapiens (Human)
样本类型：serum, plasma, ascitic fluid
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GPC5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 88-97 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 92-102 | ||||||
| 1:4 | Average % | 100 | |||||
| Range % | 94-107 | ||||||
| 1:8 | Average % | 108 | |||||
| Range % | 103-114 | ||||||
回收率：
| The recovery of human GPC5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 95-106 | |||||
| EDTA plasma (n=4) | 95 | 89-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.0512.0122.0321.847101.7881.7981.7931.60851.3741.3681.3711.1862.50.8420.8560.8490.6641.250.4940.4850.4900.3050.6250.3150.3260.3210.1360.3120.2790.2810.2800.09500.1800.1900.185 | |||||||
货期：3-5 working days
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