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产品描述：人磷脂酰肌醇聚糖4(GPC4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GPC4含量。GPC4，全称Glypican 4，是一种位于Xq26.2染色体上的基因，其编码的蛋白质属于细胞表面硫酸乙酰肝素蛋白聚糖家族。该基因在细胞分裂和生长调节中发挥作用，可能与Simpson-Golabi-Behmel综合征有关。研究机制方面，GPC4基因的表达与多种生物学过程相关，包括细胞信号传导、细胞增殖和肿瘤发生等。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于基础科研领域中对GPC4蛋白的功能机制研究，例如肿瘤微环境分析、干细胞特性探索、胚胎发育相关信号通路研究等场景，为体外实验提供稳定可靠的检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：dJ900E8.1 glypican 4 ELISA Kit; Glypican proteoglycan 4 ELISA Kit; Gpc4 ELISA Kit; GPC4_HUMAN ELISA Kit; K Glypican ELISA Kit; K-glypican ELISA Kit; Kglypican ELISA Kit; OTTHUMP00000024052 ELISA Kit; Secreted glypican 4 ELISA Kit; Secreted glypican-4 ELISA Kit; UNQ474/PRO937 ELISA Kit
缩写：GPC4
Uniprot No.：O75487
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GPC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 81-94 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-106 | |||||
回收率：
| The recovery of human GPC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-96 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.2542.3552.3052.163 201.4281.5281.4781.336 100.9350.9560.9460.804 50.5560.5670.5620.420 2.50.3720.3610.3670.225 1.250.2680.2570.2630.121 0.6250.2150.2120.2140.072 00.1420.1410.142 | ||||||
货期：3-5 working days
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