首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠磷脂酰肌醇蛋白聚糖3(GPC3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPC3含量。GPC3是一种硫酸乙酰肝素糖蛋白，在正常成人肝脏中低表达，在肝癌等多种肿瘤组织中高表达，是极具潜力的肿瘤治疗靶点。其研究机制主要围绕开发针对GPC3的单克隆抗体、CAR-T细胞疗法等，以实现对肿瘤细胞的精准杀伤。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于肝病模型研究、肿瘤微环境分析及靶向治疗机制探索等科研场景，例如可通过监测药物干预后GPC3表达水平变化评估治疗效果，或结合组织样本分析其在不同病理阶段的生物学功能。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gpc3 ELISA Kit; Glypican-3 ELISA Kit; Intestinal protein OCI-5) [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit] ELISA Kit
缩写：GPC3
Uniprot No.：P13265
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GPC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 85-99 | |||||
| 1:2 | Average % | 107 | ||||
| Range % | 97-110 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 83-93 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 91-104 | |||||
回收率：
| The recovery of rat GPC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 84-99 | ||||
| EDTA plasma (n=4) | 100 | 94-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.5062.4152.4612.2805002.0911.9742.0331.8522501.3531.3321.3431.1621250.8130.7980.8060.62562.50.4840.4560.4700.28931.20.4080.3920.4000.21915.60.2740.2670.2710.09000.1830.1790.181 | ||||||
货期：3-5 working days
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