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产品描述：人磷脂酰肌醇蛋白聚糖3(GPC-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液样本中的GPC3含量。GPC3是一种在肝癌组织中特异性高表达的蛋白聚糖，与肝癌的发生发展密切相关。其研究机制涉及调控细胞生长和分化，通过刺激致癌信号通路促进肝癌生长，并可作为肝癌诊断和治疗的新靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL，本产品广泛应用于肝癌发生机制研究、抗癌药物筛选过程中生物标志物动态监测、肿瘤微环境中GPC - 3功能解析等科研领域，为体外实验提供可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DGSX ELISA Kit; Glypican proteoglycan 3 ELISA Kit; Glypican-3 [Precursor] ELISA Kit; Gpc3 ELISA Kit; GPC3_HUMAN ELISA Kit; GTR2 2 ELISA Kit; GTR2-2 ELISA Kit; Heparan sulphate proteoglycan ELISA Kit; Intestinal protein OCI 5 ELISA Kit; Intestinal protein OCI-5 ELISA Kit; MXR7 ELISA Kit; OCI 5 ELISA Kit; OCI-5 ELISA Kit; OCI5 ELISA Kit; SDYS ELISA Kit; Secreted glypican-3 ELISA Kit; SGB ELISA Kit; SGBS ELISA Kit; SGBS1 ELISA Kit
缩写：GPC3
Uniprot No.：P51654
种属：Homo sapiens (Human)
样本类型：serum, urine, cell culture supernates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GPC-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 80-100 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human GPC-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.6212.6412.6312.437202.3562.1942.2752.081101.6261.6741.6501.45651.0211.1011.0610.8672.50.5960.6000.5980.4041.250.4120.4220.4170.2230.6250.3020.3150.3090.11500.1870.2010.194 | ||||||
货期：3-5 working days
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