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Human glypican 1 (GPC1) ELISA kit

  • 中文名： 人磷脂酰肌醇蛋白聚糖1(GPC1)酶联免疫试剂盒
货号: IPD-EL009703HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人磷脂酰肌醇蛋白聚糖1(GPC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆.样本中的GPC1含量。GPC1 即磷脂酰肌醇蛋白聚糖 -1，是细胞表面硫酸乙酰肝素蛋白聚糖家族成员。其过度表达与肿瘤发生发展相关。研究机制上，它参与肿瘤细胞增殖、迁移及血管生成等过程，还会影响肿瘤微环境，有望成为肿瘤诊断和治疗的潜在靶点。试剂盒检测范围为0.156 ng/ml-10 ng/ml.，为科研人员探索GPC1在肿瘤生物学、干细胞分化或免疫调节等领域的功能提供可靠数据。该产品严格适用于基础研究场景，例如通过检测不同处理条件下细胞或动物模型样本中的GPC1动态变化，解析其与病理生理过程的相关性，或作为潜在生物标志物的筛选工具，为后续分子机制研究奠定基础。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    FLJ38078 ELISA Kit; glypican ELISA Kit; Glypican proteoglycan 1 ELISA Kit; glypican1 ELISA Kit; GPC 1 ELISA Kit; Gpc1 ELISA Kit; GPC1_HUMAN ELISA Kit; Secreted glypican-1 ELISA Kit
  • 缩写：
    GPC1
  • Uniprot No.：
    P35052
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates.
  • 检测范围：
    0.156 ng/ml-10 ng/ml.
  • 灵敏度：
    0.039 ng/ml.
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human GPC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    96


    Range %

    90-100


    1:2

    Average %

    89


    Range %

    85-93


    1:4

    Average %

    92


    Range %

    88-96


    1:8

    Average %

    85


    Range %

    80-90


  • 回收率：

    The recovery of human GPC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    103

    99-109


    EDTA plasma (n=4)

    91

    86-97


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.267

    2.127

    2.197

    2.064


    5

    1.786

    1.875

    1.831

    1.698


    2.5

    1.295

    1.342

    1.319

    1.186


    1.25

    0.782

    0.846

    0.814

    0.681


    0.625

    0.516

    0.506

    0.511

    0.378


    0.312

    0.368

    0.352

    0.360

    0.227


    0.156

    0.273

    0.285

    0.279

    0.146


    0

    0.135

    0.131

    0.133




  • 货期：
    3-5 working days

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