首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人G蛋白偶联胆汁酸受体1(GPBAR1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的GPBAR1含量。GPBAR1（G蛋白偶联胆汁酸受体1）是一种细胞表面受体，属于G蛋白偶联受体家族，广泛分布于人体多个器官和组织中。其激活可调节炎症、能量稳态、代谢、抗凋亡和利胆等功能，与多种疾病如代谢和心血管疾病、肝胆疾病、炎症性肠病等密切相关。研究表明，GPBAR1在梗阻性黄疸的发病机制中发挥重要作用，可能成为潜在的治疗靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品可用于探究GPBAR1在胆汁酸信号通路中的调控机制，评估其在代谢性疾病动物模型或细胞实验中的表达变化，或作为药物干预研究中靶点效应的生物标志物分析工具，为肝脏代谢、炎症调控及药物开发等基础研究提供可靠的技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GPBAR1; TGR5; G-protein coupled bile acid receptor 1; G-protein coupled receptor GPCR19; hGPCR19; Membrane-type receptor for bile acids; M-BAR; hBG37; BG37
缩写：GPBAR1
Uniprot No.：Q8TDU6
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GPBAR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 93-102 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 85-92 | ||||||
| 1:4 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 85-94 | ||||||
回收率：
| The recovery of human GPBAR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 95-105 | |||||
| EDTA plasma (n=4) | 88 | 84-92 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 102.3392.2042.2722.161 51.9511.9921.9721.861 2.51.3631.4021.3831.272 1.250.7060.6980.7020.591 0.6250.3480.3520.3500.239 0.3120.2500.2570.2540.143 0.1560.2010.1980.2000.089 00.1120.1100.111 | |||||||
货期：3-5 working days
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