首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血小板膜糖蛋白ⅡbⅢa(GP-ⅡbⅢa/CD41+CD61)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPⅡbⅢa/CD41+CD61含量。GPⅡbⅢa/CD41+CD61作为靶点，在血小板功能中至关重要。参与血小板聚集过程，研究机制聚焦于通过阻断该靶点，抑制血小板的聚集与黏附，进而为抗血栓药物研发提供方向，以防治相关心血管疾病。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于多种生物样本中GP - ⅡbⅢa的精确检测，可广泛用于大鼠模型中血小板功能调控机制研究、血栓相关疾病动物模型的病理分析、药物干预效果评价等科研领域，为探索血小板相关分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：platelet membrane glycoprotein ⅡbⅢa,GP-ⅡbⅢa |platelet membrane glycoprotein 2b3a,GP-2b3a
缩写：GPⅡbⅢa/CD41+CD61
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GP-ⅡbⅢa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 82-95 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of rat GP-ⅡbⅢa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 98 | 92-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.1762.0532.1151.9212001.8551.7641.8101.6161001.5421.4691.5061.312501.2361.1781.2071.013250.8370.7860.8120.61812.50.4210.3930.4070.2136.250.2130.1960.2050.01100.1980.1900.194 | ||||||
货期：3-5 working days
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