| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 62 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human GAB4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GAB4抗体的模拟参考文献示例(注：部分信息为假设性概括，实际文献需通过学术数据库验证)：
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1. **文献名称**：*GRB2-associated binding protein 4 (GAB4) as a prognostic marker in breast cancer: antibody validation and clinical significance*
**作者**：Smith A, et al.
**摘要**：本研究开发并验证了一种特异性GAB4抗体，用于检测乳腺癌组织中GAB4的过表达。结果显示，GAB4与HER2信号通路激活相关，其高表达与患者不良预后显著相关。
2. **文献名称**：*GAB4 antibody-based detection of tyrosine kinase signaling complexes in lung adenocarcinoma*
**作者**：Chen L, et al.
**摘要**：通过免疫共沉淀和Western blot技术，利用GAB4抗体揭示了其在非小细胞肺癌中与EGFR信号通路的相互作用，表明GAB4可能成为靶向治疗的潜在生物标志物。
3. **文献名称**：*Development of a monoclonal antibody against GAB4 for functional studies in neuronal cells*
**作者**：Tanaka K, et al.
**摘要**：本文报道了一种高特异性抗GAB4单克隆抗体的制备，并验证其在神经元细胞中的亚定位及功能，提示GAB4可能在神经突触可塑性中发挥作用。
4. **文献名称**：*GAB4 expression in autoimmune diseases: an immunohistochemical analysis using novel antibody probes*
**作者**：Garcia R, et al.
**摘要**：通过新型GAB4抗体对类风湿关节炎患者组织进行免疫组化分析，发现GAB4在炎症细胞中的异常表达可能参与自身免疫反应的调控机制。
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**提示**：以上为模拟文献，如需真实文献，建议通过PubMed、Google Scholar等平台检索关键词“GAB4 antibody”或“GRB2-associated binding protein 4”，并筛选抗体开发、应用或机制研究相关论文。
**Background of GAB4 Antibody**
The GAB4 antibody targets the Grb2-associated binding protein 4 (GAB4), a member of the GAB family of scaffolding/adaptor proteins involved in intracellular signal transduction. GAB4. like its homologs GAB1. GAB2. and GAB3. plays a role in mediating signals from growth factor receptors (e.g., EGFR, insulin receptor) and cytokine receptors by recruiting downstream effectors through multiple phosphorylation sites and protein interaction domains. Structurally, GAB4 contains a pleckstrin homology (PH) domain, which facilitates membrane localization, and tyrosine residues that, when phosphorylated, serve as docking sites for SH2 domain-containing proteins like GRB2 or PI3K.
GAB4 antibodies are primarily used in research to study its expression, localization, and post-translational modifications in cellular signaling pathways. Studies suggest GAB4 is involved in immune regulation, neuronal development, and cancer progression, though its functions remain less characterized compared to other GAB proteins. Dysregulation of GAB4 has been linked to pathologies such as leukemia and autoimmune diseases. In experimental settings, GAB4 antibodies enable detection via Western blotting, immunoprecipitation, or immunofluorescence, aiding investigations into its role in receptor tyrosine kinase (RTK) signaling networks and potential therapeutic targeting. Current research focuses on elucidating its tissue-specific functions and interactions within signaling cascades to better understand its contributions to health and disease.
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