关键信息
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基因名
STAT3
- 应用
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别名
STAT3; signal transducer and activator of transcription 3 (acute-phase response factor); signal transducer and activator of transcription 3; APRF
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种属
Human
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表达系统
E. coli
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标签
N-terminal His-Tag
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P40763
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表达区间
127-688aa
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氨基酸序列
GQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLK SQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKT LTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQ QKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTG VQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNM EESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGL KIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQV AEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKG FSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSE SSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNIL VSPLVYLYPDIPKEEAFGKYCR
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分子量
67.7kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Signal Transducer and Activator of Transcription 3 (STAT3) is a critical transcription factor involved in various cellular processes, including proliferation, survival, and differentiation. Dysregulation of STAT3 activity has been implicated in numerous diseases, particularly in cancers, where its persistent activation contributes to tumorigenesis and the survival of malignant cells. Research on recombinant STAT3 proteins has gained momentum due to their potential therapeutic applications and role in elucidating the pathways related to cell signaling and cancer progression. Scientists have employed various expression systems, such as bacterial, yeast, and mammalian cells, to produce functional STAT3 proteins, enabling the study of its structure, dynamics, and interactions with other cellular molecules. Understanding the mechanisms by which STAT3 is activated and functions can provide insights into novel drug targets and therapeutic strategies. Furthermore, the development of STAT3 inhibitors is of considerable interest in cancer treatment, as they may offer a way to impede aberrant signaling pathways. Overall, the study of recombinant STAT3 proteins is essential for advancing our knowledge of its biological significance and exploring new avenues for clinical intervention.
