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Rabbit Polyclonal ULK2 Antibody

  • 中文名: ULK2抗体
  • 别    名: ATG1B; Unc51.2
货号: IPDX12918
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/25-1/50 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesATG1B; Unc51.2
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenSynthetic peptide of human ULK2
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于ULK2抗体的参考文献示例(注:以下为示例性内容,实际引用需核实文献真实性):

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1. **"ULK1 and ULK2 Regulate Autophagy Initiation"**

*作者:Mizushima N. et al. (2010)*

**摘要**:研究揭示了ULK1和ULK2在自噬启动中的关键作用,通过特异性抗体检测其在哺乳动物细胞中的表达和亚细胞定位,证实了二者在营养缺乏时的功能差异。

2. **"Characterization of ULK2 Antibodies for Autophagy Research"**

*作者:Egan D.F. et al. (2015)*

**摘要**:系统评估了多种ULK2抗体的特异性,利用基因敲除细胞系验证抗体有效性,为自噬相关研究提供了可靠的检测工具(Western blot和免疫荧光应用)。

3. **"ULK2 Expression Correlates with Prognosis in Colorectal Cancer"**

*作者:Kundu M. et al. (2018)*

**摘要**:通过免疫组化分析ULK2在结直肠癌组织中的表达,发现其高表达与患者不良预后相关,所用抗体经预吸附实验验证特异性。

4. **"A Novel Monoclonal Antibody for ULK2 Detection in Human Tissues"**

*作者:Smith J. et al. (2020)*

**摘要**:报道了一种新开发的ULK2单克隆抗体,通过质谱和敲除细胞验证其高特异性,适用于多种样本类型(如冰冻组织和肿瘤细胞系)。

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建议通过PubMed或Google Scholar以“ULK2 antibody”、“ULK2 autophagy”等关键词检索最新文献以获取准确信息。

背景信息

The ULK2 antibody is a crucial tool in studying the Unc-51-like kinase 2 (ULK2), a serine/threonine kinase involved in autophagy initiation. ULK2. along with ULK1. forms a conserved kinase complex that regulates autophagosome formation under nutrient-deprived conditions. These kinases integrate upstream signals from mTORC1 and AMPK pathways to orchestrate cellular responses to energy stress. While ULK1 is more extensively characterized, ULK2 shares structural homology and partially overlapping functions, with potential compensatory roles in autophagy when ULK1 is absent. The ULK2 antibody enables detection of ULK2 protein expression, post-translational modifications (e.g., phosphorylation at specific residues), and subcellular localization via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Research applications span cancer biology, neurodegenerative diseases, and metabolic disorders, as ULK2 dysregulation is implicated in tumor progression, neuronal survival, and nutrient sensing. Recent studies also explore ULK2's non-autophagic roles in membrane trafficking and apoptosis. Commercial antibodies typically target conserved regions like the N-terminal kinase domain or C-terminal divergent sequences, requiring validation via knockout controls. Its utility extends to therapeutic development, particularly in autophagy-modulating strategies for disease treatment.

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