| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HER4; ALS19; p180erbB4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ERBB4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于ERBB4抗体的代表性文献摘要整理：
1. **文献名称**：*ERBB4 structural analysis and antibody validation in breast cancer subtypes*
**作者**：Jones et al.
**摘要**：研究通过X射线晶体学解析ERBB4受体结构，并验证多种商业抗体在乳腺癌细胞系中的特异性，发现部分抗体存在非特异性结合问题，推荐使用C-terminal磷酸化特异性抗体(如HFR1)进行功能研究。
2. **文献名称**：*ERBB4 antibody-based detection of somatic mutations in lung adenocarcinoma*
**作者**：Wang & Chen
**摘要**：开发新型ERBB4单克隆抗体(克隆号4B2)，成功应用于免疫组化检测非小细胞肺癌组织中的ERBB4胞外域突变，证实其突变状态与EGFR抑制剂治疗响应相关。
3. **文献名称**：*Neuregulin-ERBB4 signaling in cortical interneuron development*
**作者**：Fazzari et al.
**摘要**：使用兔源多克隆ERBB4抗体(Abcam#ab32370)进行脑组织免疫荧光，揭示ERBB4在小鼠前脑中间神经元迁移中的关键作用，抗体特异性通过基因敲除模型验证。
注：以上文献信息为模拟示例，实际引用需核对真实出版物。建议通过PubMed或Web of Science搜索最新研究，重点关注抗体验证方法(如敲除/敲除实验、表位定位)和应用场景(Western blot/IHC/流式细胞术)。
The ERBB4 antibody is a crucial tool for studying the ERBB4 receptor, a member of the epidermal growth factor receptor (EGFR) tyrosine kinase family. ERBB4. also known as HER4. plays diverse roles in cellular processes, including proliferation, differentiation, and survival, by binding to ligands like neuregulins. Structurally, it consists of an extracellular ligand-binding domain, a transmembrane region, and an intracellular tyrosine kinase domain. Unlike other EGFR members, ERBB4 undergoes unique proteolytic cleavage, releasing a soluble intracellular domain that translocates to the nucleus to regulate gene expression.
ERBB4 is implicated in both physiological and pathological contexts. It is essential for heart development, neurogenesis, and mammary gland differentiation. However, its role in cancer is complex, acting as a tumor suppressor in some contexts (e.g., melanoma) or an oncogene in others (e.g., breast cancer). Dysregulation of ERBB4 signaling is also linked to neuropsychiatric disorders like schizophrenia and bipolar disorder.
ERBB4 antibodies are widely used in research to detect ERBB4 expression, localization, and activation states via techniques such as Western blotting, immunohistochemistry, and flow cytometry. They enable the identification of ERBB4 isoforms and post-translational modifications, aiding studies on receptor dimerization, downstream signaling (e.g., PI3K/AKT, MAPK pathways), and therapeutic targeting. Specific clones or monoclonal antibodies are validated for applications in cell lines, tissues, or biofluids, ensuring precise detection in disease models or clinical samples. These antibodies are pivotal in unraveling ERBB4's dual roles in health and disease.
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