| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP3R2 |
| Uniprot No | Q96LZ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-173aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MSTMGNEASY PAEMCSHFDN DEIKRLGRRF KKLDLDKSGS LSVEEFMSLP ELRHNPLVRR VIDVFDTDGD GEVDFKEFIL GTSQFSVKGD EEQKLRFAFS IYDMDKDGYI SNGELFQVLK MMVGNNLTDW QLQQLVDKTI IILDKDGDGK ISFEEFSAVV RDLEIHKKLV LIV |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP3R2重组蛋白的3篇代表性文献示例(注：文献为示例性质，具体内容需根据实际检索结果调整)：
---
1. **文献名称**：*Structural and functional characterization of recombinant PPP3R2 in calcineurin signaling*
**作者**：Li, X. et al.
**摘要**：研究通过在大肠杆菌中表达并纯化重组PPP3R2蛋白，解析其与钙调磷酸酶催化亚基(PPP3CA)的相互作用机制，揭示PPP3R2通过特定结构域调控磷酸酶活性的分子基础。
2. **文献名称**：*PPP3R2 regulates neuronal calcium signaling via recombinant protein interaction assays*
**作者**：Wang, Y. et al.
**摘要**：利用重组PPP3R2蛋白进行体外结合实验，证明其在神经细胞中通过结合Ca²⁺/钙调蛋白复合物，调节钙调磷酸酶活性，进而影响突触可塑性与学习记忆功能。
3. **文献名称**：*Expression and purification of human PPP3R2 recombinant protein for antibody development*
**作者**：Garcia, R. et al.
**摘要**：报道了一种高效表达和纯化人源PPP3R2重组蛋白的方法，并利用该蛋白成功制备特异性抗体，为相关疾病的诊断工具开发提供了材料基础。
---
**备注**：以上文献为示例，实际文献需通过PubMed、Google Scholar等平台以关键词“PPP3R2 recombinant protein”或“PPP3R2 expression”检索获取。建议结合最新研究补充具体信息。
PPP3R2 (protein phosphatase 3 regulatory subunit B2), also known as calcineurin B subunit type 2. is a regulatory component of the calcium/calmodulin-dependent protein phosphatase calcineurin (PP2B). This serine/threonine phosphatase plays a critical role in calcium-mediated signaling pathways, particularly in neuronal function, immune response, and cardiac development. The PPP3R2 protein binds to the catalytic subunit PPP3CA/PPP3CB, forming an active heterodimer that dephosphorylates target proteins to regulate processes like T-cell activation, synaptic plasticity, and apoptosis.
Recombinant PPP3R2 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional regulatory subunits for research applications. Its recombinant form typically retains the ability to bind calcium ions via EF-hand motifs and interact with calcineurin catalytic subunits. Researchers utilize PPP3R2 recombinant proteins to study calcineurin activation mechanisms, screen inhibitors for immunosuppressive drug development, or model calcium signaling disruptions in neurological disorders. Unlike the ubiquitously expressed PPP3R1 (CNB1), PPP3R2 shows tissue-specific expression patterns, with higher abundance in testis and brain tissues, suggesting specialized roles in these systems. Structural studies using recombinant PPP3R2 have helped clarify isoform-specific differences in calcineurin regulation and their implications in disease pathways, including Alzheimer’s and cardiovascular conditions.
×
