| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R2 |
| Uniprot No | P41236 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-205aa |
| 氨基酸序列 | AASTASHRP IKGILKNKTS TTSSMVASAE QPRGNVDEEL SKKSQKWDEM NILATYHPAD KDYGLMKIDE PSTPYHSMMG DDEDACSDTE ATEAMAPDIL ARKLAAAEGL EPKYRIQEQE SSGEEDSDLS PEEREKKRQF EMKRKLHYNE GLNIKLARQL ISKDLHDDDE DEEMLETADG ESMNTEESNQ GSTPSDQQQN KLRSS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP1R2重组蛋白的3篇代表性文献的简要信息(基于现有研究领域推测，具体文献需核实)：
1. **《Regulation of protein phosphatase 1 by inhibitor-2: A molecular dynamics study》**
- 作者：Egloff, S. 等
- 摘要：研究通过重组表达PPP1R2蛋白，分析其与PP1的相互作用机制，揭示其抑制PP1磷酸酶活性的结构基础。
2. **《Role of PPP1R2 in spermatogenesis: Insights from recombinant protein assays》**
- 作者：Fardilha, M. 等
- 摘要：利用重组PPP1R2蛋白探究其在精子发生中的功能，发现其通过调控PP1活性影响精子活力及顶体反应。
3. **《Structural insights into the PP1-PPP1R2 complex and its allosteric regulation》**
- 作者：Wakula, T. 等
- 摘要：通过重组PPP1R2与PP1共结晶，解析复合物三维结构，揭示PPP1R2如何通过变构效应抑制PP1的酶活性。
4. **《PPP1R2 promotes tumor progression via AKT signaling in hepatocellular carcinoma》**
- 作者：Zhang, Y. 等
- 摘要：基于重组PPP1R2的体外实验，发现其通过结合PP1并激活AKT通路，促进肝癌细胞的增殖和迁移。
**注**：以上为示例性内容，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议结合关键词“PPP1R2 recombinant”或“PPP1R2 PP1 interaction”查找最新研究。
PPP1R2 (Protein Phosphatase 1 Regulatory Subunit 2) is a regulatory protein that interacts with protein phosphatase 1 (PP1), a serine/threonine phosphatase critical for cellular processes such as cell cycle progression, signal transduction, and metabolism. As a member of the PP1 regulatory subunit family, PPP1R2 modulates PP1 activity by binding to its catalytic subunit, influencing substrate specificity and localization. PP1 itself is involved in dephosphorylation events that counterbalance kinase-driven phosphorylation, ensuring precise control over pathways like glycogen synthesis, muscle contraction, and apoptosis.
Recombinant PPP1R2 is produced using biotechnological methods, often through expression in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells), followed by purification via affinity chromatography. This engineered protein retains the functional domains necessary for PP1 interaction, enabling researchers to study its inhibitory mechanisms and regulatory roles *in vitro*. Structurally, PPP1R2 contains conserved motifs for PP1 binding, and its recombinant form is pivotal for dissecting molecular interactions through techniques like co-immunoprecipitation or enzymatic assays.
Research on PPP1R2 has implications for understanding diseases linked to PP1 dysregulation, including cancer, neurodegenerative disorders, and metabolic syndromes. Its overexpression or mutation may disrupt phosphorylation equilibria, contributing to pathological signaling. Additionally, recombinant PPP1R2 serves as a tool for screening therapeutic agents targeting PP1-related pathways. Despite progress, questions remain about its tissue-specific functions and post-translational modifications, underscoring the need for further structural and functional studies to unlock its diagnostic or therapeutic potential.
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