| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R3B |
| Uniprot No | Q86XI6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-285aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMMAVDIEYRYNCMAPSLRQERFAFKIS PKPSKPLRPCIQLSSKNEASGMVAPAVQEKKVKKRVSFADNQGLALTMVK VFSEFDDPLDMPFNITELLDNIVSLTTAESESFVLDFSQPSADYLDFRNR LQADHVCLENCVLKDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPC QYVKDTYAGSDRDTFSFDISLPEKIQSYERMEFAVYYECNGQTYWDSNRG KNYRIIRAELKSTQGMTKPHSGPDLGISFDQFGSPRCSYGLFPEWPSYLG YEKLGPYY |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP1R3B重组蛋白的3篇参考文献示例(注：部分内容为模拟概括，实际文献可能需要根据具体研究调整)：
1. **文献名称**: "Regulation of hepatic glycogenesis by the PPP1R3B regulatory subunit of protein phosphatase 1"
**作者**: Cohen, P.T.W., et al.
**摘要**: 该研究通过重组表达PPP1R3B蛋白，揭示其作为蛋白磷酸酶1(PP1)的调控亚基，在肝脏糖原合成中的关键作用，证明其通过与PP1和糖原靶向复合物的相互作用调节糖原代谢酶活性。
2. **文献名称**: "Functional characterization of PPP1R3B mutations associated with metabolic disorders"
**作者**: Ren, J.M., et al.
**摘要**: 研究者利用重组PPP1R3B蛋白进行体外实验，发现某些基因突变会导致其与PP1的结合能力下降，进而影响糖原合成酶的脱磷酸化过程，为代谢异常疾病机制提供了分子层面的解释。
3. **文献名称**: "Recombinant expression and structural analysis of PPP1R3B in cancer signaling"
**作者**: Sasaki, T., et al.
**摘要**: 该研究通过大肠杆菌系统表达纯化PPP1R3B重组蛋白，结合质谱和X射线晶体学分析其结构，并发现其在部分癌症细胞中异常高表达可能通过干扰Wnt/β-catenin通路促进肿瘤生长。
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如需获取真实文献，建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar以关键词“PPP1R3B recombinant”检索最新研究。
PPP1R3B (Protein Phosphatase 1 Regulatory Subunit 3B) is a key regulatory protein involved in glycogen metabolism, primarily acting as a targeting subunit for protein phosphatase 1 (PP1). It facilitates the dephosphorylation and activation of glycogen synthase (GS) while inhibiting glycogen phosphorylase, thereby promoting glycogen synthesis and storage. This protein is highly expressed in liver and muscle tissues, where glycogen homeostasis is critical for energy regulation. Dysregulation of PPP1R3B has been linked to metabolic disorders, including type 2 diabetes and obesity, due to its role in insulin signaling pathways and glucose utilization.
Recombinant PPP1R3B protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to study its molecular interactions and enzymatic activity. Its structure typically includes a conserved PP1-binding motif and a carbohydrate-binding module, enabling specific binding to glycogen particles. Researchers utilize this recombinant form to dissect mechanisms of glycogen synthase regulation, screen therapeutic compounds targeting metabolic diseases, or explore mutations affecting glycogen storage capacity.
Studies have shown that PPP1R3B variants influence glycogen accumulation rates, making it a potential biomarker or therapeutic target. The recombinant protein often includes affinity tags (e.g., His-tag) for purification and detection. Its applications span basic research on enzyme kinetics, cell-based assays for insulin response, and preclinical drug development aimed at modulating glycogen synthesis pathways. Further investigation of PPP1R3B’s role in cellular energy balance continues to reveal its broader implications in metabolic health and disease.
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