| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R1A |
| Uniprot No | P30153 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-589aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAAADGDDSLYPIAVLIDELRNEDVQL RLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFT TLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAH FVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDD TPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEA CVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPE ITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQIL PCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDEC PEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYM PLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEW AHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLR MAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKY FAQEALTVLSLA |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与PPP2R1A重组蛋白相关的3篇文献摘要信息：
1. **文献名称**: "Recurrent mutations in the PP2A regulatory subunit PPP2R1A in endometrial and ovarian carcinomas"
**作者**: Jespersen, C. et al.
**摘要**: 该研究在子宫内膜癌和卵巢癌中鉴定了PPP2R1A基因的反复体细胞突变，发现这些突变导致PP2A复合体功能异常，可能通过干扰细胞周期调控和凋亡通路促进肿瘤发生。
2. **文献名称**: "Structural insights into PP2A regulatory subunit B56α (PPP2R5A) function through recombinant protein studies"
**作者**: Seshacharyulu, P. et al.
**摘要**: 通过重组表达PPP2R1A与B56α亚基，解析了PP2A全酶复合体的互作机制，发现PPP2R1A作为支架蛋白对底物特异性及亚细胞定位具有关键调控作用。
3. **文献名称**: "Targeting PPP2R1A in HER2-positive breast cancer cells restores PP2A activity and inhibits tumor growth"
**作者**: Kaur, A. et al.
**摘要**: 利用重组PPP2R1A蛋白重建PP2A活性，发现其能抑制HER2阳性乳腺癌细胞的增殖并增强化疗敏感性，提示其作为潜在治疗靶点的价值。
The PPP2R1A gene encodes the scaffolding Aα subunit of protein phosphatase 2A (PP2A), a critical serine/threonine phosphatase involved in regulating diverse cellular processes, including cell cycle progression, signal transduction, and apoptosis. PP2A exists as a heterotrimeric complex comprising a structural scaffolding subunit (A; e.g., PPP2R1A), a regulatory subunit (B), and a catalytic subunit (C). PPP2R1A serves as a platform for assembling distinct PP2A holoenzymes, determining substrate specificity and subcellular localization. Its modular structure, featuring 15 HEAT repeats, facilitates interactions with B subunits and other regulatory proteins.
Recombinant PPP2R1A protein is generated using expression systems (e.g., E. coli, mammalian cells) to study PP2A assembly, function, and interactions. This engineered protein often includes affinity tags (e.g., His, GST) for purification and detection. Research highlights PPP2R1A's role in maintaining PP2A activity and its dysregulation in diseases. Somatic mutations in PPP2R1A (e.g., Arg182 substitutions) are linked to cancers, including endometrial and lung cancers, impairing PP2A's tumor-suppressive functions by disrupting holoenzyme assembly or substrate binding. Additionally, PPP2R1A expression alterations are associated with neurodegenerative disorders and metabolic syndromes.
Recombinant PPP2R1A enables mechanistic studies, such as mapping interaction domains, analyzing mutant effects, and screening for therapeutic agents targeting PP2A-related pathways. Its use in structural biology (e.g., cryo-EM) has advanced understanding of PP2A complex dynamics. However, post-translational modifications (e.g., phosphorylation, methylation) in native PPP2R1A may differ in recombinant forms, necessitating cautious interpretation. Overall, PPP2R1A recombinant proteins are vital tools for elucidating PP2A's roles in health and disease.
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