| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP3R1 |
| Uniprot No | P63098 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-170aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGNEASYPLEMCSHFDADEIKRLGKRFKKL DLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVS QFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQ QIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与PPP3R1重组蛋白相关的3篇参考文献示例(注：内容为模拟生成，实际文献需通过学术数据库验证)：
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1. **标题**: *Structural and functional characterization of the recombinant human PPP3R1 regulatory subunit*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过大肠杆菌表达系统重组表达了人源PPP3R1蛋白，并解析其与钙调神经磷酸酶催化亚基(PPP3CA)的相互作用机制。实验表明，重组PPP3R1可增强磷酸酶活性，并在钙离子信号通路中起关键调控作用。
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2. **标题**: *PPP3R1 mutations impair T-cell receptor signaling by disrupting calcineurin regulatory function*
**作者**: Li H, et al.
**摘要**: 通过构建PPP3R1重组蛋白突变体，揭示了其在T细胞受体信号传导中的功能缺陷。研究发现，特定突变导致PPP3R1与钙调蛋白结合能力下降，从而抑制钙调神经磷酸酶活性，影响NFAT通路激活。
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3. **标题**: *Recombinant PPP3R1 as a therapeutic target for cardiac hypertrophy*
**作者**: Wang X, et al.
**摘要**: 利用哺乳动物细胞表达系统获得高纯度PPP3R1重组蛋白，验证其在心肌肥厚模型中的调控作用。实验表明，PPP3R1通过调节钙调神经磷酸酶-NFAT轴，抑制病理性心肌细胞肥大。
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如需获取真实文献，建议在 **PubMed** 或 **Web of Science** 中搜索关键词“PPP3R1 recombinant”“PPP3R1 expression”或结合研究领域(如“calcineurin signaling”“NFAT pathway”)。
**Background of PPP3R1 Recombinant Protein**
PPP3R1. also known as calcineurin subunit B type 1. is a regulatory subunit of the calcium/calmodulin-dependent serine/threonine phosphatase calcineurin. This protein plays a critical role in calcium-mediated signaling pathways, particularly in immune response, neuronal function, and cardiac development. Structurally, PPP3R1 contains four EF-hand calcium-binding motifs, enabling it to sense intracellular calcium fluctuations and modulate calcineurin activity by binding to its catalytic subunit (PPP3CA or PPP3CB). This interaction is essential for the phosphatase activity of calcineurin, which dephosphorylates substrates like NFAT (nuclear factor of activated T-cells), triggering downstream transcriptional responses.
Recombinant PPP3R1 protein is engineered for research applications, often produced in *E. coli* or mammalian expression systems with tags (e.g., His-tag) for purification. Its production enables studies on calcineurin’s role in cellular processes, including T-cell activation, synaptic plasticity, and hypertrophy. Dysregulation of PPP3R1 or calcineurin is linked to pathologies such as cardiac hypertrophy, autoimmune disorders, and neurodegenerative diseases. Recombinant PPP3R1 is also utilized in drug discovery, particularly for immunosuppressants (e.g., cyclosporine A) that target calcineurin, as well as in structural studies to map binding interfaces with regulatory partners like FKBP or CABIN1. Additionally, it aids in characterizing mutations associated with PPP3R1-related genetic disorders, providing insights into molecular mechanisms and therapeutic strategies.
In summary, PPP3R1 recombinant protein serves as a vital tool for dissecting calcineurin signaling, disease mechanisms, and developing targeted therapies.
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