| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R8 |
| Uniprot No | Q12972 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-351aa |
| 氨基酸序列 | MAAAANSGSS LPLFDCPTWA GKPPPGLHLD VVKGDKLIEK LIIDEKKYYL FGRNPDLCDF TIDHQSCSRV HAALVYHKHL KRVFLIDLNS THGTFLGHIR LEPHKPQQIP IDSTVSFGAS TRAYTLREKP QTLPSAVKGD EKMGGEDDEL KGLLGLPEEE TELDNLTEFN TAHNKRISTL TIEEGNLDIQ RPKRKRKNSR VTFSEDDEII NPEDVDPSVG RFRNMVQTAV VPVKKKRVEG PGSLGLEESG SRRMQNFAFS GGLYGGLPPT HSEAGSQPHG IHGTALIGGL PMPYPNLAPD VDLTPVVPSA VNMNPAPNPA VYNPEAVNEP KKKKYAKEAW PGKKPTPSLL IVEHHHHHH |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP1R8重组蛋白的3篇模拟参考文献，涵盖其功能、结构及相互作用研究：
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1. **标题**: *"Recombinant PPP1R8 (NIPP1) regulates PP1 activity through a novel binding interface"*
**作者**: Van Duyne, G.D., et al.
**摘要**: 本研究利用重组PPP1R8蛋白，揭示了其通过C端结构域与蛋白磷酸酶PP1的相互作用机制，并证明其调控PP1在核内的定位及底物特异性，为理解细胞周期调控提供了新视角。
2. **标题**: *"Structural insights into the interaction of PPP1R8 with splicing factor complexes"*
**作者**: Jagiello, I., et al.
**摘要**: 通过表达纯化重组PPP1R8蛋白并结合X射线晶体学，研究发现其N端结构域直接结合剪接因子SAP18/SAP30.揭示了PPP1R8在pre-mRNA加工及表观遗传调控中的双重作用。
3. **标题**: *"Expression and functional characterization of recombinant PPP1R8 in DNA damage response"*
**作者**: Li, H., & Wang, Y.
**摘要**: 该研究在大肠杆菌中高效表达重组PPP1R8蛋白，发现其通过招募PP1至DNA损伤位点促进修复，并验证了其磷酸化修饰对修复通路的关键调控作用。
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**注**：以上文献为模拟示例，实际引用需以具体数据库检索结果为准。建议通过PubMed或Web of Science以“PPP1R8 recombinant”“NIPP1 PP1 interaction”等关键词查找最新文献。
PPP1R8 (Protein Phosphatase 1 Regulatory Subunit 8), also known as NIPP1 (Nuclear Inhibitor of Protein Phosphatase 1), is a regulatory protein that interacts with protein phosphatase 1 (PP1), a critical enzyme involved in dephosphorylation and cellular signaling. Structurally, PPP1R8 contains an N-terminal RVxF motif for PP1 binding and C-terminal PP1-inhibitory domains, along with RNA-binding regions. It plays dual roles: modulating PP1 activity and participating in RNA processing, chromatin remodeling, and cell cycle regulation.
Recombinant PPP1R8 is produced in vitro using expression systems like *E. coli* or mammalian cells, often tagged for purification (e.g., His-tag). Its production enables functional studies of PP1 regulation, particularly in contexts like DNA damage response, pre-mRNA splicing, and ribosome biogenesis. Research highlights its involvement in maintaining genomic stability and epigenetic regulation, with aberrant PPP1R8 expression linked to cancers and neurodegenerative disorders.
The recombinant protein is widely used to investigate PP1-dependent pathways, protein-RNA interactions, and therapeutic targeting. For example, studies explore how PPP1R8’s inhibition of PP1 affects mitotic progression or how its RNA-binding activity influences gene expression. Its role as a scaffold protein bridging PP1 with other molecules (e.g., splicing factors) also makes it a focus in understanding cellular stress responses. Current challenges include elucidating tissue-specific functions and context-dependent post-translational modifications.
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