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产品描述:人糖蛋白激素α链(CGA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织培养上清液样本中的CGA含量。试剂盒检测范围为4 mIU/mL-120 mIU/mL,适用于生殖医学研究、内分泌功能探索、激素分泌机制分析等科研领域,支持科研人员高效完成激素相关分子机理研究或细胞模型中的蛋白分泌动态监测。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Anterior pituitary glycoprotein hormones common subunit alpha ELISA Kit; CG alpha ELISA Kit; CG-alpha ELISA Kit; cga ELISA Kit; Choriogonadotropin alpha chain ELISA Kit; Chorionic Gonadotrophin Alpha ELISA Kit; Chorionic Gonadotrophin Alpha Subunit ELISA Kit; Chorionic gonadotrophin subunit alpha ELISA Kit; chorionic gonadotropin, alpha polypeptide ELISA Kit; Follicle-stimulating hormone alpha ELISA Kit; Follicle-stimulating hormone alpha chain ELISA Kit; follicle-stimulating hormone alpha subunit ELISA Kit; Follitropin alpha ELISA Kit; Follitropin alpha chain ELISA Kit; FSH Alpha ELISA Kit; FSH-alpha ELISA Kit; FSHA ELISA Kit; GLHA_HUMAN ELISA Kit; Glycoprotein hormones alpha chain ELISA Kit; Glycoprotein Hormones Alpha Polypeptide ELISA Kit; GPH Alpha ELISA Kit; GPHa ELISA Kit; GPHA1 ELISA Kit; HCG ELISA Kit; hyrotropin alpha ELISA Kit; LH-alpha ELISA Kit; LHA ELISA Kit; LSH alpha ELISA Kit; LSH-alpha ELISA Kit; Luteinizing hormone alpha ELISA Kit; Luteinizing hormone alpha chain ELISA Kit; Lutropin alpha ELISA Kit; Lutropin alpha chain ELISA Kit; Thyroid Stimulating Hormone Alpha ELISA Kit; Thyroid Stimulating Hormone Alpha Chain ELISA Kit; Thyroid-stimulating hormone alpha chain ELISA Kit; Thyrotropin alpha chain ELISA Kit; TSH Alpha ELISA Kit; TSH-alpha ELISA Kit; TSHA ELISA Kit
缩写:CGA
Uniprot No.:P01215
种属:Homo sapiens (Human)
样本类型:serum, plasma, cell culture supernates
检测范围:4 mIU/mL-120 mIU/mL
灵敏度:1 mIU/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cancer
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<15% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CGA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 92 | |||||
Range % | 89-98 | ||||||
1:2 | Average % | 95 | |||||
Range % | 88-99 | ||||||
1:4 | Average % | 94 | |||||
Range % | 90-101 | ||||||
1:8 | Average % | 95 | |||||
Range % | 86-100 |
回收率:
The recovery of human CGA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 95 | 91-100 | |||||
EDTA plasma (n=4) | 96 | 91-99 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
mIU/mlOD1OD2AverageCorrected 1202.5252.5012.5132.466 501.2991.3231.3111.264 200.6200.6130.6170.570 80.3050.3170.3110.264 40.1570.1520.1550.108 00.0460.0480.047 |
货期:3-5 working days
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