Rabbit Polyclonal UBB Antibody

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Hela cells, mouse liver tissue, Primary antibody: P06953(UBB Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds    

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P06953(UBB Antibody) at dilution 1/20. (Original magnification: ×200)    

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P06953(UBB Antibody) at dilution 1/20. (Original magnification: ×200)    

Ubiquitin B (UBB) is a member of the ubiquitin protein family, which plays a central role in protein degradation through the ubiquitin-proteasome system (UPS). As a small regulatory protein, ubiquitin is covalently attached to target proteins via a process called ubiquitination, marking them for proteasomal degradation. UBB, encoded by the UBB gene in humans (located on chromosome 17p11.2), is notable for containing tandem repeats of ubiquitin-coding sequences. These repeats can lead to the production of polyubiquitin chains under stress conditions, serving as a reservoir of ubiquitin monomers to maintain cellular homeostasis.

UBB antibodies are essential tools for studying ubiquitination dynamics, protein turnover, and UPS-related pathologies. They enable the detection of UBB expression, ubiquitin-conjugated proteins, or polyubiquitin chains in various experimental setups, including Western blotting, immunohistochemistry, and immunofluorescence. Aberrant UBB expression or mutations have been linked to neurodegenerative diseases (e.g., Alzheimer’s and Parkinson’s) due to impaired protein clearance and accumulation of toxic aggregates. UBB antibodies also aid in cancer research, as dysregulated ubiquitination is associated with tumor progression and drug resistance.

When using UBB antibodies, specificity is critical, as cross-reactivity with other ubiquitin isoforms (e.g., UBA, UBC) or homologs can occur. Researchers often validate antibodies using knockout controls or peptide blocking assays to ensure accurate results. Both monoclonal and polyclonal UBB antibodies are available, each offering distinct advantages depending on the application.