首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人囊性纤维化跨膜传导调节蛋白(CFTR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CFTR含量。CFTR即囊性纤维化跨膜传导调节因子。它的基因突变会引发囊性纤维化疾病。探究CFTR蛋白的结构与功能，了解其在氯离子和碳酸氢根离子转运中的作用，为开发治疗囊性纤维化的药物和疗法提供依据。试剂盒检测范围为28 pg/mL-1800 pg/mL，在科研场景中，可用于探索CFTR蛋白在细胞离子通道调控、黏液分泌异常等病理过程中的作用，或用于评估基因编辑、药物干预等实验条件下CFTR表达水平的变化，为囊性纤维化相关基础研究及潜在治疗策略开发提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ABC 35 ELISA Kit; ABC35 ELISA Kit; ABCC 7 ELISA Kit; ABCC7 ELISA Kit; ATP binding cassette sub family C member 7 ELISA Kit; ATP Binding Cassette Superfamily C Member 7 ELISA Kit; ATP binding cassette transporter sub family C member 7 ELISA Kit; ATP-binding cassette sub-family C member 7 ELISA Kit; cAMP dependent chloride channel ELISA Kit; cAMP-dependent chloride channel ELISA Kit; CF ELISA Kit; CFTR ELISA Kit; CFTR/MRP ELISA Kit; CFTR_HUMAN ELISA Kit; Channel conductance controlling ATPase ELISA Kit; Channel conductance-controlling ATPase ELISA Kit; Cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub family C; member 7) ELISA Kit; Cystic fibrosis transmembrane conductance regulator ELISA Kit; Cystic fibrosis transmembrane conductance regulator ATP binding cassette sub family C member 7 ELISA Kit; Cystic Fibrosis Transmembrane Regulator ELISA Kit; dJ760C5.1 ELISA Kit; MRP 7 ELISA Kit; MRP7 ELISA Kit; TNR CFTR ELISA Kit
缩写：CFTR
Uniprot No.：P13569
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：28 pg/mL-1800 pg/mL
灵敏度：7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CFTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human CFTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-106 | ||||
| EDTA plasma (n=4) | 91 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected18001.9562.1102.0331.8879001.5451.6221.5841.4384501.2041.2861.2451.0992250.9030.9310.9170.771112.50.5310.5110.5210.375560.3370.3420.3400.194280.2480.2580.2530.10700.1410.1510.146 | ||||||
货期：3-5 working days
×
