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产品描述:山羊促甲状腺素(TSH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CGA含量。试剂盒检测范围为0.5 μIU/mL-20 μIU/mL,该产品适用于动物科学领域的基础研究,包括甲状腺功能异常模型建立、环境应激对内分泌系统的影响评估,以及畜牧业中营养干预与生殖调控相关的激素水平监测,为深入探索山羊甲状腺 - 垂体轴调控机制提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:CGAGlycoprotein hormones alpha chain ELISA Kit; Anterior pituitary glycoprotein hormones common subunit alpha ELISA Kit; Follicle-stimulating hormone alpha chain ELISA Kit; FSH-alpha ELISA Kit; Follitropin alpha chain ELISA Kit; Luteinizing hormone alpha chain ELISA Kit; LSH-alpha ELISA Kit; Lutropin alpha chain ELISA Kit; Thyroid-stimulating hormone alpha chain ELISA Kit; TSH-alpha ELISA Kit; Thyrotropin alpha chain ELISA Kit
缩写:CGA
Uniprot No.:Q8WMW8
种属:Capra hircus (Goat)
样本类型:serum, plasma, tissue homogenates
检测范围:0.5 μIU/mL-20 μIU/mL
灵敏度:0.25 μIU/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cancer
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of goat TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 86 | ||||
Range % | 82-92 | |||||
1:2 | Average % | 94 | ||||
Range % | 91-98 | |||||
1:4 | Average % | 102 | ||||
Range % | 97-105 | |||||
1:8 | Average % | 92 | ||||
Range % | 84-98 |
回收率:
The recovery of goat TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 90-97 | ||||
EDTA plasma (n=4) | 105 | 102-109 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
μIU/mlOD1OD2Average202.1222.2782.200101.2381.2041.22150.6520.6630.6582.50.3680.3470.35810.1530.1580.1560.50.0830.0810.082 |
货期:3-5 working days
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