首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人丝切蛋白1(CFL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CFL1含量。CFL1是重要的靶点。它影响细胞骨架重塑，参与细胞运动等过程。研究发现其异常表达与肿瘤等疾病的发生发展相关。围绕它的研究机制多聚焦于调控其活性的信号通路，旨在通过干预CFL1为相关疾病治疗提供新思路。试剂盒检测范围为25 pg/mL-1600 pg/mL，适用于体外培养细胞模型、动物组织或体液样本中CFL1蛋白表达的定量分析，可应用于细胞生物学、癌症研究、神经科学等领域的基础科研，能满足不同实验场景下对低丰度靶蛋白的检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：18 kDa phosphoprotein ELISA Kit; CFL 1 ELISA Kit; CFL ELISA Kit; CFL1 ELISA Kit; COF1_HUMAN ELISA Kit; Cofilin 1 ELISA Kit; Cofilin 1 non muscle ELISA Kit; Cofilin ELISA Kit; Cofilin non muscle isoform ELISA Kit; Cofilin-1 ELISA Kit; epididymis secretory protein Li 15 ELISA Kit; HEL-S-15 ELISA Kit; non-muscle isoform ELISA Kit; p18 ELISA Kit
缩写：CFL1
Uniprot No.：P23528
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CFL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 98-105 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 95-00 | ||||||
| 1:8 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
回收率：
| The recovery of human CFL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 90-96 | |||||
| EDTA plasma (n=4) | 105 | 101-109 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16002.6042.6382.6212.4868002.0782.0412.0601.9254001.3291.3051.3171.1822000.8040.7970.8010.6661000.4310.4560.4440.309500.2940.2870.2910.156250.2130.2060.2100.07500.1360.1340.135 | |||||||
货期：3-5 working days
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