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产品描述：人补体因子H相关蛋白3(CFHR3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CFHR3含量。人补体因子H相关蛋白3（CFHR3）是一种补体调节蛋白，与补体系统功能密切相关。其基因变异与肾脏疾病如非典型溶血性尿毒症综合征和C3肾小球病有关，通过调节补体功能影响疾病发生发展。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，本产品可广泛应用于体外实验研究，包括补体系统功能解析、疾病模型建立中的生物标志物检测以及免疫调节相关分子机制探索等科研场景，为探究CFHR3在炎症反应、免疫复合物清除等过程中的动态变化提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CFHR3 ELISA kit; CFHL3 ELISA kit; FHR3Complement factor H-related protein 3 ELISA kit; FHR-3 ELISA kit; DOWN16 ELISA kit; H factor-like protein 3 ELISA kit
缩写：CFHR3
Uniprot No.：Q02985
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 ng/mL-2000 ng/mL
灵敏度：7.8 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CFHR3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:200 | Average % | 101 | ||||
| Range % | 97-104 | |||||
| 1:400 | Average % | 96 | ||||
| Range % | 89-99 | |||||
| 1:800 | Average % | 98 | ||||
| Range % | 93-101 | |||||
回收率：
| The recovery of human CFHR3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-94 | ||||
| EDTA plasma (n=4) | 93 | 90-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected20002.6232.6322.6282.53210001.8141.7881.8011.7055000.9880.9630.9760.8802500.5710.5540.5630.4671250.3340.3470.3410.24562.50.2610.2530.2570.16131.250.1530.1660.1600.06400.0970.0950.096 | ||||||
货期：3-5 working days
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