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产品描述：人补体因子I(CFI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CFI含量。试剂盒检测范围为6.25 ng/mL-400 ng/mL，本试剂盒基于高特异性抗体对目标蛋白的捕获与检测，实验流程标准化，可满足科研场景下对CFI表达的定量需求，例如探究补体系统相关疾病的分子机制、评估炎症或免疫紊乱模型中CFI的动态变化，或用于组织微环境中补体调控因子的基础研究。试剂盒支持多种生物样本类型，为体外实验提供灵活选择，适用于细胞生物学、免疫学及病理生理学等领域的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AHUS3 ELISA Kit; ARMD13 ELISA Kit; C3b INA ELISA Kit; C3b inactivator ELISA Kit; C3B/C4B inactivator ELISA Kit; C3BINA ELISA Kit; CFAI_HUMAN ELISA Kit; Cfi ELISA Kit; Complement component I ELISA Kit; Complement control protein factor I ELISA Kit; Complement factor I ELISA Kit; Complement factor I heavy chain ELISA Kit; Complement factor I light chain ELISA Kit; F1 ELISA Kit; factor I ELISA Kit; FactorI ELISA Kit; FI ELISA Kit; I factor ELISA Kit; IF ELISA Kit; KAF ELISA Kit; Konglutinogen activating factor ELISA Kit; Light chain of factor I ELISA Kit; OTTHUMP00000219728 ELISA Kit; OTTHUMP00000221928 ELISA Kit
缩写：CFI
Uniprot No.：P05156
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CFI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 89-94 | |||||
| 1:400 | Average % | 101 | ||||
| Range % | 98-103 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 89-96 | |||||
| 1:1600 | Average % | 97 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of human CFI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 92-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.7802.7662.7732.6352002.2882.3112.3002.1621001.7431.6841.7141.576501.1141.1011.1080.970250.6220.6520.6370.49912.50.3450.3360.3410.2036.250.2370.2240.2310.09300.1400.1360.138 | ||||||
货期：3-5 working days
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