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Mouse Advanced glycosylation end product-specific receptor(AGER) ELISA kit

  • 中文名: 小鼠晚期糖基化终产物特异性受体(AGER)ELISA kit
货号: IPD-EL001441MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠晚期糖基化终产物特异性受体(AGER)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆.样本中的AGER含量。AGER 即晚期糖基化终末产物受体,在炎症反应、氧化应激等病理过程中起重要作用。其研究机制主要聚焦于配体与其结合后激活下游信号通路,参与调控细胞增殖、凋亡等。对它的研究有助于深入了解相关疾病发病机制,为开发新疗法提供靶点。试剂盒检测范围为0.312 ng/ml-20 ng/ml,适用于基础医学研究中AGER在糖尿病肾病、阿尔茨海默病模型或心血管疾病中的表达水平分析,也可用于体外实验(如细胞培养上清)中AGER的动态变化监测,为探索疾病分子机制或药物干预效果评估提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Ager ELISA Kit; Rage ELISA Kit; Advanced glycosylation end product-specific receptor ELISA Kit; Receptor for advanced glycosylation end products ELISA Kit
  • 缩写:
    AGER
  • Uniprot No.:
    Q62151
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.312 ng/ml-20 ng/ml
  • 灵敏度:
    0.078 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse AGER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    102


    Range %

    98-108


    1:2

    Average %

    107


    Range %

    100-114


    1:4

    Average %

    92


    Range %

    88-97


    1:8

    Average %

    98


    Range %

    91-104


  • 回收率:

    The recovery of mouse AGER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    102

    97-108


    EDTA plasma (n=4)

    95

    90-100


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    3.042

    2.934

    2.988

    2.836


    10

    2.433

    2.367

    2.400

    2.248


    5

    1.705

    1.728

    1.717

    1.565


    2.5

    1.059

    1.029

    1.044

    0.892


    1.25

    0.638

    0.621

    0.629

    0.477


    0.625

    0.403

    0.383

    0.393

    0.241


    0.312

    0.277

    0.284

    0.280

    0.128


    0

    0.155

    0.149

    0.152




  • 货期:
    3-5 working days

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