首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人蛋白聚糖(AGC1)酶联免疫试剂盒,为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AGC1含量。AGC1（Aggrecan）是一种在软骨中发挥关键作用的蛋白聚糖，参与软骨结构和关节功能。其研究机制涉及软骨细胞中蛋白酶体功能受损，导致SOX9和AGC1 mRNA水平降低，可能成为骨关节炎的治疗靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品支持科研人员探究AGC1在骨关节炎、类风湿性关节炎等病理模型中的动态变化，或用于评估组织工程中细胞外基质的重塑机制，为软骨相关疾病的分子机制研究及生物标志物筛选提供可靠工具。其高灵敏度和特异性设计使其兼容多种研究场景，包括体外细胞实验、动物模型分析及临床前样本的定量检测，严格适用于生命科学研究领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ACAN ELISA Kit; AGC1 ELISA Kit; CSPG1 ELISA Kit; MSK16 ELISA Kit; Aggrecan core protein ELISA Kit; Cartilage-specific proteoglycan core protein ELISA Kit; CSPCP ELISA Kit; Chondroitin sulfate proteoglycan core protein 1 ELISA Kit; Chondroitin sulfate proteoglycan 1) [Cleaved into: Aggrecan core protein 2] ELISA Kit
缩写：AGC1
Uniprot No.：P16112
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AGC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 89 | |||||
| Range % | 83-94 | ||||||
| 1:10 | Average % | 100 | |||||
| Range % | 95-105 | ||||||
| 1:20 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:40 | Average % | 89 | |||||
| Range % | 85-91 | ||||||
回收率：
| The recovery of human AGC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 105 | 100-110 | |||||
| EDTA plasma (n=4) | 88 | 83-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.4842.4312.4582.28152.0682.1042.0861.9092.51.4101.5001.4551.2781.250.7830.7980.7910.6140.6250.4840.5010.4930.3160.3120.3420.3350.3390.1620.1560.2390.2410.2400.06300.1780.1760.177 | |||||||
货期：3-5 working days
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