首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠甲种胎儿球蛋白/甲胎蛋白(AFP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的AFP含量。AFP即甲胎蛋白，是一种糖蛋白，主要由胎儿肝细胞及卵黄囊合成。在成人，AFP升高可能与肝癌、生殖细胞肿瘤等恶性肿瘤相关。研究机制上，它可作为肿瘤标志物辅助诊断、监测病情。其产生机制可能与肿瘤细胞的异常表达有关。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品可广泛应用于肝癌动物模型研究、干细胞分化监测、组织再生机制探索及肿瘤相关信号通路分析等科研领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AfpAlpha-fetoprotein ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit
缩写：AFP
Uniprot No.：P02773
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:40 | Average % | 89 | ||||
| Range % | 82-94 | |||||
| 1:80 | Average % | 101 | ||||
| Range % | 96-108 | |||||
| 1:160 | Average % | 94 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of rat AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-94 | ||||
| EDTA plasma (n=4) | 97 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.7012.6312.6662.4785002.2392.2122.2262.0382501.7101.6631.6871.4991251.0971.1741.1360.94862.50.6510.6950.6730.48531.20.4270.4590.4430.25515.60.3370.3170.3270.13900.1920.1840.188 | ||||||
货期：3-5 working days
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