首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Afamin(AFM)酶联免疫试剂盒,为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AFM含量。试剂盒检测范围为3.9 ng/mL-250 ng/mL，适用于科研领域对AFM在代谢调控、肝脏功能评估及肿瘤微环境等方向的研究；为探索AFM在脂质代谢异常、卵巢癌生物标志物筛选或慢性炎症相关疾病中的分子机制提供可靠工具，尤其适用于基础医学研究、疾病模型构建及药物干预效果评价等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AFAM_HUMAN ELISA Kit; Afamin ELISA Kit; Afm ELISA Kit; ALB 2 ELISA Kit; ALB2 ELISA Kit; ALBA ELISA Kit; ALF ELISA Kit; Alpha albumin ELISA Kit; Alpha-Alb ELISA Kit; Alpha-albumin ELISA Kit
缩写：AFM
Uniprot No.：P43652
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.9 ng/mL-250 ng/mL
灵敏度：0.97 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human AFM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2000 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4000 | Average % | 100 | ||||
| Range % | 95-106 | |||||
| 1:8000 | Average % | 97 | ||||
| Range % | 92-104 | |||||
回收率：
| The recovery of human AFM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 93-96 | ||||
| EDTA plasma (n=4) | 102 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2502.0242.0982.0611.9291251.7151.7611.7381.60662.51.3341.3641.3491.21731.250.8930.8840.8890.75715.60.5670.5460.5570.4257.80.3210.3490.3350.2033.90.2610.2460.2540.12200.1310.1330.132 | ||||||
货期：3-5 working days
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