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产品描述：猪甲胎蛋白(AFP)酶联免疫试剂盒,为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的AFP含量。AFP即甲胎蛋白，是一种糖蛋白。它在胎儿早期由肝脏和卵黄囊合成。在肝癌等多种恶性肿瘤中会异常升高，临床上常用作肿瘤标志物辅助诊断。研究机制集中于其表达调控及与肿瘤细胞增殖、转移等过程的关联，以探寻新的治疗靶点。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于猪模型研究中肝脏疾病机制探索、肿瘤发生发展监测、生殖生理学研究等科研领域，满足体外实验中对AFP蛋白表达水平动态变化的精准追踪需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AFPAlpha-fetoprotein ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit
缩写：AFP
Uniprot No.：Q8MJ76
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates, cell culture supernates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 88-102 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 82-94 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of pig AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 91 | 86-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.4162.3522.3842.2891001.6831.6611.6721.577500.9450.9040.9250.830250.4920.4590.4760.38112.50.3020.2870.2950.2006.250.2140.2090.2120.1173.120.1510.1450.1480.05300.0980.0920.095 | ||||||
货期：3-5 working days
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