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产品描述：人β肾上腺素能受体激酶1(ADRBK1)酶联免疫试剂盒,为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ADRBK1含量。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，该产品为研究ADRBK1在心血管疾病、代谢综合征或神经调节等领域的分子机制提供可靠工具，支持体外实验中对药物干预、基因编辑或病理模型下蛋白表达水平的定量评估，适用于基础科研中信号通路解析、生物标志物探索及分子互作研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADRBK1 ELISA Kit; Adrenergic beta receptor kinase 1 ELISA Kit; ARBK1_HUMAN ELISA Kit; BARK ELISA Kit; BARK1 ELISA Kit; Beta adrenergic receptor kinase 1 ELISA Kit; Beta ARK 1 ELISA Kit; Beta ARK1 ELISA Kit; Beta-adrenergic receptor kinase 1 ELISA Kit; Beta-ARK-1 ELISA Kit; FLJ16718 ELISA Kit; G protein coupled receptor kinase 2 ELISA Kit; G-protein coupled receptor kinase 2 ELISA Kit; GRK2 ELISA Kit
缩写：ADRBK1
Uniprot No.：P25098
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADRBK1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 91 | |
| Range % | 88-94 | ||
| 1:2 | Average % | 95 | |
| Range % | 90-101 | ||
| 1:4 | Average % | 97 | |
| Range % | 94-102 | ||
| 1:8 | Average % | 94 | |
| Range % | 91-99 |
回收率：
| The recovery of human ADRBK1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-93 | ||||
| EDTA plasma (n=4) | 99 | 93-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 12002.0362.1662.1011.927 6001.3731.4051.3891.215 3000.9970.9570.9770.803 1500.6330.6750.6540.480 750.3920.4020.3970.223 37.50.2940.2840.2890.115 18.750.2410.2330.2370.063 00.1670.1800.174 | ||||||
货期：3-5 working days
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