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产品描述：人金属肽酶含血小板反应蛋白2(ADAMTS2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADAMTS2含量。ADAMTS2是一种细胞外金属蛋白酶，参与细胞外基质破坏、血管平滑肌细胞分型与凋亡、炎症细胞浸润、氧化应激等复杂机制。其在心脏肥厚、肝纤维化等疾病中发挥重要作用，通过调节PI3K/Akt信号通路影响心肌细胞肥大反应。试剂盒检测范围为3.12 ng/mL-200 ng/mL，支持科研人员探究 ADAMTS2 在皮肤弹性维持、器官纤维化病理机制或肿瘤微环境调控中的作用，为疾病模型构建、药物靶点筛选及生物标志物研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A disintegrin and metalloproteinase with thrombospondin motifs 2 ELISA Kit; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 2 ELISA Kit; ADAM metallopeptidase with thrombospondin type 1 motif 2 ELISA Kit; ADAM TS 2 ELISA Kit; ADAM TS2 ELISA Kit; ADAM-TS 2 ELISA Kit; ADAM-TS2 ELISA Kit; ADAMTS 3 ELISA Kit; ADAMTS-2 ELISA Kit; ADAMTS2 ELISA Kit; ATS2_HUMAN ELISA Kit; EC 3.4.24.14 ELISA Kit; EDS VIIB ELISA Kit; EDS VIIC ELISA Kit; hPCPNI ELISA Kit; NPI ELISA Kit; PC I NP ELISA Kit; PC I-NP ELISA Kit; PCINP ELISA Kit; PCPNI ELISA Kit; pNPI ELISA Kit; Procollagen I N proteinase ELISA Kit; Procollagen I N-proteinase ELISA Kit; Procollagen I/II amino propeptide processing enzyme ELISA Kit; Procollagen I/II amino propeptide-processing enzyme ELISA Kit; Procollagen N endopeptidase ELISA Kit; Procollagen N-endopeptidase ELISA Kit
缩写：ADAMTS2
Uniprot No.：O95450
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-95 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
回收率：
| The recovery of human ADAMTS2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 98-109 | |||||
| EDTA plasma (n=4) | 97 | 92-101 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected2002.1292.2382.1842.0231001.7371.6821.7101.549501.1121.1741.1430.982250.7190.7410.7300.56912.50.4800.4590.4700.3096.250.3600.3500.3550.1943.120.2390.2470.2430.08200.1590.1630.161 | |||||||
货期：3-5 working days
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