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产品描述：人解整合素样金属蛋白酶9(ADAM9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液、腹水样本中的ADAM9含量。ADAM9 是一种具有重要研究意义的靶点。它在多种生理和病理过程中发挥作用，例如参与细胞黏附、迁移等。科研人员围绕其作用机制开展研究，试图明晰其在疾病发生发展中的具体角色，为开发针对性治疗策略奠定基础。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于体外研究ADAM9在肿瘤微环境调控、细胞外基质重塑及疾病模型中的表达动态，为探索其分子机制提供可靠工具。实验结果仅用于科学研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADAM 9 ELISA Kit; ADAM metallopeptidase domain 9 ELISA Kit; Adam9 ELISA Kit; ADAM9_HUMAN ELISA Kit; Cellular disintegrin-related protein ELISA Kit; Cone rod dystrophy 9 ELISA Kit; CORD9 ELISA Kit; Disintegrin and metalloproteinase domain-containing protein 9 ELISA Kit; MCMP ELISA Kit; MDC9 ELISA Kit; Meltrin-gamma ELISA Kit; Metalloprotease/disintegrin/cysteine-rich protein 9 ELISA Kit; Mltng ELISA Kit; Myeloma cell metalloproteinase ELISA Kit
缩写：ADAM9
Uniprot No.：Q13443
种属：Homo sapiens (Human)
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 9-101 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 84-92 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-97 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human ADAM9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-93 | ||||
| EDTA plasma (n=4) | 93 | 89-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.6572.5572.6072.445 20001.9241.8241.8741.712 10001.1261.1161.1210.959 5000.6550.6450.6500.488 2500.3800.3700.3750.213 1250.2710.2610.2660.104 62.50.2150.2120.2140.052 00.1630.1610.162 | ||||||
货期：3-5 working days
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